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リコンビナントRabMAb

Anti-SorLA/SORL1 抗体 [EPR23262-4] (BSA and Azide free) (ab302509)

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Immunocytochemistry/ Immunofluorescence - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (ab302509)
  • Immunocytochemistry/ Immunofluorescence - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (ab302509)
  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)
  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)
  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)
  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)
  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)
  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)
  • Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23262-4] to SorLA/SORL1 - BSA and Azide free
  • Suitable for: IHC-Fr, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free)
    SorLA/SORL1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23262-4] to SorLA/SORL1 - BSA and Azide free

  • 由来種

    Rabbit

  • 特異性

    ICC application is not suitable for rat.

  • アプリケーション

    適用あり: IHC-Fr, ICC/IFmore details
    適用なし: Flow Cyt (Intra),IHC-P,IP or WB

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-Fr: Mouse and rat cerebrum, lung, skeletal muscle frozen (Fresh) tissues. ICC/IF: SK-BR-3 and mouse primary neural/glia cells.

  • 特記事項

    ab302509 is the carrier-free version of ab302508.

     

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302509の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra),IHC-P,IP or WB.

ターゲット情報

  • 機能

    Likely to be a multifunctional endocytic receptor, that may be implicated in the uptake of lipoproteins and of proteases. Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. Binds the receptor-associated protein (RAP). Could play a role in cell-cell interaction.

  • 組織特異性

    Expressed mainly in brain, where it is most abundant in the cerebellum, cerebral cortex and the occipital pole; low expression in the putamen and the thalamus. According to PubMed:9157966, found in spinal cord, testis, liver, kidney and pancreas with detectable levels in placenta, lung and heart. According to PubMed:8940146, expressed in the prostate, ovary, thyroid and spleen, but not found in kidney, liver, lung, skeletal muscle, bone marrow and adrenals.

  • 配列類似性

    Belongs to the SORL1 family.
    Contains 5 BNR repeats.
    Contains 1 EGF-like domain.
    Contains 6 fibronectin type-III domains.
    Contains 11 LDL-receptor class A domains.
    Contains 5 LDL-receptor class B repeats.

  • 翻訳後修飾

    The propeptide removed in the N-terminus may be cleaved by furin or homologous proteases.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • C11orf32 antibody
    • FLJ21930 antibody
    • FLJ39258 antibody
    • gp250 antibody
    • LDLR relative with 11 ligand binding repeats antibody
    • LDLR relative with 11 ligand-binding repeats antibody
    • Low density lipoprotein receptor relative with 11 ligand binding repeats antibody
    • Low-density lipoprotein receptor relative with 11 ligand-binding repeats antibody
    • LR 11 antibody
    • LR11 antibody
    • LRP 9 antibody
    • LRP9 antibody
    • Mosaic protein LR11 antibody
    • SORL 1 antibody
    • SORL_HUMAN antibody
    • SORL1 antibody
    • SorLA 1 antibody
    • SorLA antibody
    • SorLA-1 antibody
    • Sortilin related receptor antibody
    • Sortilin related receptor L(DLR class) A repeats containing antibody
    • Sortilin-related receptor antibody
    • Sorting protein related receptor containing LDLR class A repeats antibody
    • Sorting protein-related receptor containing LDLR class A repeats antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (ab302509)
    Immunocytochemistry/ Immunofluorescence - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (ab302509)

    This data was developed using AB302508, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cells labeling SorLA/SORL1 with AB302508 at 1/50 dilution (10.32 µ/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed antibody at 1/1000 dilution (2µ/mL) (Green). Confocal image showing cytoplasmic staining in mouse primary neural/glia cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Negative control: NIH/3T3 (PMID: 11082041) . ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4µ/mL dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Primary diluent was used instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed at 1/1000 dilution (2µ/mL).

  • Immunocytochemistry/ Immunofluorescence - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (ab302509)
    Immunocytochemistry/ Immunofluorescence - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (ab302509)

    This data was developed using AB302508, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-BR-3 (human breast adenocarcinoma epithelial cell) cells labeling SorLA/SORL1 with AB302508 at 1/50 dilution (10.32 µ/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed antibody at 1/1000 dilution (2µ/mL) (Green). Confocal image showing cytoplasmic staining in SK-BR-3 cell line. Negative control: MDA-MB-231 (PMID: 31138794) . ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5µ/mL) (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Primary diluent was used instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed at 1/1000 dilution (2µ/mL).

  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)
    Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)

    This data was developed using AB302508, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle (fresh) tissue labeling SorLA/SORL1 with AB302508 at 1/500 (1.03(2 µg/mL)) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Negative control: no staining on rat skeletal muscle (PMID:9157966) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).

  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)
    Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)

    This data was developed using AB302508, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat lung (fresh) tissue labeling SorLA/SORL1 with AB302508 at 1/500 (1.03µg/mL) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Negative control: no staining on rat lung (PMID:9157966) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).

  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)
    Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)

    This data was developed using AB302508, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labeling SorLA/SORL1 with AB302508 at 1/500 (1.03µg/mL) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).

  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)
    Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)

    This data was developed using AB302508, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labeling SorLA/SORL1 with AB302508 at 1/500 (1.03µg/mL) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Negative control: no staining on mouse skeletal muscle (PMID:9157966) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).

  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)
    Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)

    This data was developed using AB302508, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung (fresh) tissue labeling SorLA/SORL1 with AB302508 at 1/500 (1.03 µg/mL) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Negative control: no staining on mouse lung (PMID:9157966) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).

  • Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)
    Immunohistochemistry (Frozen sections) - Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)

    This data was developed using AB302508, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling SorLA/SORL1 with AB302508 at 1/500 (1.03µg/mL) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).

  • Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)
    Anti-SorLA/SORL1 antibody [EPR23262-4] (BSA and Azide free) (AB302509)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab302509 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab302509 は論文での使用が確認できていません。

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