Anti-SORBS1 抗体

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SORBS1 antibody (AB4551)

ab4551 (2.5 μg/ml) staining SORBS1 in Human Skeletal Muscle by IHC-P. Steamed antigen retrieval in citrate buffer pH6, AP-staining. This image shows spotty staining on muscle fibres in longitudinal section.

• WB

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Western blot - Anti-SORBS1 antibody (AB4551)

ab4551 was tested in in human plasma, human white blood cells and HEK293 cells. Samples were run for 1h at 200 V using the Fisherbrand vertical complete gel unit, and transferred onto nitrocellulose 0.45um (1h, 100V, 4 C) using the Bio-rad Trans blot cell with plate electrodes. Membranes were blocked for 1 h at RT, then incubated overnight in primary antibody (1/200, 1/1000, 1/5000 or minus primary antibody) at 4 degrees C, with shaking. Following washing, membranes were incubated in secondary antibody (1/5000, Alexa-Fluor680 donkey anti-goat IgG, H+L) for 1 h at RT. Antibody binding was detected using LI-COR Bioscience's Odyssey Infrared Imaging system (intensity 5). The molecular weight of sorbs 1 was calculated using 'Compute pI/MW'from ExPASy tools based on the amino acid sequence according to NP_006425. The band indicated is approximately this predicted molecular weight, and is not present in the minus primary image, scanned at the same intensity. Image courtesy of an anonymous abreview.

All lanes:

Western blot - Anti-SORBS1 antibody (ab4551)

Lane 1:

Molecular weight marker (Bio-Rad Precision Plus Kaleidoscope standards)

Lane 2:

10 μl Human plasma 1 (600-800 μg protein)

Lane 3:

10 μl Human plasma 2 (600-800 μg protein)

Lane 4:

10 μl Human White Blood cells 1 (30-50 μg protein)

Lane 5:

10 μl Human White Blood cells 2 (30-50 μg protein)

Lane 6:

10 μl HEK293 whole cell lysate (prepared by scrapping 10 cm confluent dish into 4ml of 2x sample buffer (Sigma)

Secondary

All lanes:

Donkey anti-goat IgG (H+L) conjugated to Alexa-Fluor680 at 1/5000 dilution

Predicted band size: 142 kDa

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