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リコンビナントRabMAb

Anti-Sodium/calcium exchanger 2 抗体 [EPR25106-37] - BSA and Azide free (ab300456)

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Western blot - Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free (AB300456)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free (AB300456)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free (AB300456)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free (AB300456)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free (AB300456)
  • Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free (AB300456)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25106-37] to Sodium/calcium exchanger 2
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free
    Sodium/calcium exchanger 2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25106-37] to Sodium/calcium exchanger 2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-Fr or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human hypothalamus, human cerebellum, human liver, mouse brain, mouse striatum, mouse spleen, mouse liver and rat striatum lysates. IHC-P: Human cerebrum, rat cerebrum, and mouse cerebrum tissues.

  • 特記事項

    ab300456 is the carrier-free version of ab300455.

     

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Constituent: 100% PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR25106-37

  • アイソタイプ

    IgG

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300456の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr or IP.

ターゲット情報

  • 機能

    Rapidly transports Ca(2+) during excitation-contraction coupling. Ca(2+) is extruded from the cell during relaxation so as to prevent overloading of intracellular stores.

  • 配列類似性

    Belongs to the sodium/potassium/calcium exchanger family. SLC8 subfamily.
    Contains 2 Calx-beta domains.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Na(+)/Ca(2+)-exchange protein 2 antibody
      • NAC2_HUMAN antibody
      • SLC8A2 antibody
      • Sodium/calcium exchanger 2 antibody
      • Solute carrier family 8 member 2 antibody
      see all

    画像

    • Western blot - Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free (AB300456)
      Western blot - Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free (AB300456)
      All lanes : Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] (ab300455) at 1/1000 dilution

      Lane 1 : Human hypothalamus tissue lysate at 20 µg
      Lane 2 : Human cerebellum tissue lysate at 20 µg
      Lane 3 : Human liver tissue lysate at 40 µg
      Lane 4 : Mouse brain tissue lysate at 20 µg
      Lane 5 : Mouse striatum tissue lysate at 20 µg
      Lane 6 : Mouse spleen tissue lysate at 20 µg
      Lane 7 : Mouse liver tissue lysate at 40 µg
      Lane 8 : Rat striatum tissue lysate at 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Observed band size: 100-120,240,60 kDa why is the actual band size different from the predicted?



      This data was developed using 300455, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Samples are non-boiled as boiling may cause protein aggregates.

      The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 10767288, 24641279).

      Low expression: human liver, mouse spleen, mouse liver(PMID: 8798769).

      Exposure time: 26 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free (AB300456)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free (AB300456)

      This data was developed using ab300455, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling Sodium/calcium exchanger 2 with ab300455 at 1/100 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection kit). Positive staining on human cerebrum (PMID: 12818181, PMID: 12722103). The section was incubated with ab300455 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by a ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free (AB300456)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free (AB300456)

      This data was developed using ab300455, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling Sodium/calcium exchanger 2 with ab300455 at 1/2000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection kit). Positive staining on rat cerebrum (PMID: 12818181, PMID: 12722103). The section was incubated with ab300455 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by a ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free (AB300456)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free (AB300456)

      This data was developed using ab300455, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling Sodium/calcium exchanger 2 with ab300455 at 1/2000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection kit). Positive staining on mouse cerebrum (PMID: 12818181, PMID: 12722103). The section was incubated with ab300455 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by a ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free (AB300456)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free (AB300456)

      This data was developed using ab300455, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human liver tissue labelling Sodium/calcium exchanger 2 with ab300455 at 1/2000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection kit). Negative control: no staining on human liver (PMID: 12722103). The section was incubated with ab300455 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by a ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free (AB300456)
      Anti-Sodium/calcium exchanger 2 antibody [EPR25106-37] - BSA and Azide free (AB300456)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300456 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300456 は論文での使用が確認できていません。

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