Anti-SOD2/MnSOD 抗体 [EPR2560Y] (ab68155)
![Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)](https://www.abcam.co.jp/ps/products/68/ab68155/Images/ab68155-5-anti-sod2-antibody-epr2560y-western-blot-human-lysate.jpg "Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2560Y] to SOD2/MnSOD
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SOD2/MnSOD antibody [EPR2560Y]
SOD2/MnSOD 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2560Y] to SOD2/MnSOD -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human SOD2/MnSOD aa 1-100 (N terminal). The exact sequence is proprietary.
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ポジティブ･コントロール
- WB: SH-SY5Y, A549, HEK-293T and HAP1 cell lysates; Human heart tissue; Rat brain and heart lysate. IHC-P: Human liver tissue sections. IHC-Fr: Rat lung tissue sections.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2560Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68155の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. -
関連疾患
Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
配列類似性
Belongs to the iron/manganese superoxide dismutase family. -
翻訳後修飾
Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 6648 Human
- Entrez Gene: 20656 Mouse
- Entrez Gene: 24787 Rat
- Omim: 147460 Human
- SwissProt: P04179 Human
- SwissProt: P09671 Mouse
- SwissProt: P07895 Rat
- Unigene: 487046 Human
see all -
別名
- Indophenoloxidase B antibody
- IPO B antibody
- IPOB antibody
see all
画像
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Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)All lanes : Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) at 1/1000 dilution
Lane 1 : SHSY5Y cell lysate
Lane 2 : A549 cell lysate
Lane 3 : Wild-type HEK-293T cell lysate
Lane 4 : SOD2 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 25 kDaLanes 1 - 4: Merged signal (red and green). Green - ab68155 observed at 22 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab68155 was shown to react with SOD2/MnSOD in wild-type HEK-293T. Loss of signal was observed when knockout cell line ab255434 (knockout cell lysate ab263835) was used. Wild-type and SOD2/MnSOD knockout samples were subjected to SDS-PAGE. ab68155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)](https://www.abcam.co.jp/ps/products/68/ab68155/Images/ab68155-261350-ab68155SOD2.jpg)
Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)All lanes : Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : SOD2 knockout HAP1 cell lysate
Lane 3 : Human heart tissue lysate
Lane 4 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 25 kDaLanes 1 - 4: Merged signal (red and green). Green - ab68155 observed at 29 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab68155 was shown to specifically react with SOD2 when SOD2 knockout samples were used. Wild-type and SOD2 knockout samples were subjected to SDS-PAGE. ab68155 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)](https://www.abcam.co.jp/ps/products/68/ab68155/Images/ab68155_1.jpg)
Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)All lanes : Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) at 1/500 dilution
Lane 1 : Rat brain lysate
Lane 2 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)](https://www.abcam.co.jp/ps/products/68/ab68155/Images/ab68155_3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)ab68155 at 1:100 dilution staining Superoxide Dismutase 2 in human liver tissue sections.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)](https://www.abcam.co.jp/ps/products/68/ab68155/Images/ab68155-6-benefits-of-recombinant-antibodies.png)
Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (79)
ab68155 は 79 報の論文で使用されています。
- Sun X et al. Mitochondrial transplantation ameliorates doxorubicin-induced cardiac dysfunction via activating glutamine metabolism. iScience 26:107790 (2023). WB ; Human . PubMed: 37731615
- Viguier C et al. Impact of physical activity on brain oxidative metabolism and intrinsic capacities in young swiss mice fed a high fat diet. Neuropharmacology 241:109730 (2023). WB ; Mouse . PubMed: 37758019
- Wang T et al. Spatiotemporal Regulation of Injectable Heterogeneous Silk Gel Scaffolds for Accelerating Guided Vertebral Repair. Adv Healthc Mater 12:e2202210 (2023). PubMed: 36465008
- Scisciola L et al. Targeting high glucose-induced epigenetic modifications at cardiac level: the role of SGLT2 and SGLT2 inhibitors. Cardiovasc Diabetol 22:24 (2023). PubMed: 36732760
- Lian CY et al. Persistent activation of Nrf2 in a p62-dependent non-canonical manner aggravates lead-induced kidney injury by promoting apoptosis and inhibiting autophagy. J Adv Res 46:87-100 (2023). PubMed: 37003700