Anti-SOD2/MnSOD 抗体 (ab118340)
Key features and details
- Goat polyclonal to SOD2/MnSOD
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-SOD2/MnSOD antibody
SOD2/MnSOD 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to SOD2/MnSOD -
由来種
Goat -
特異性
ab118340 is expected to recognize both reported isoforms (NP_000627.2; NP_001019637.1). The immunizing peptide represents the acetylation site including K122 according to isoform A. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human, Pig
交差が予測される動物種: Cow, Dog, Zebrafish
-
免疫原
Synthetic peptide corresponding to Human SOD2/MnSOD aa 100-200 (internal sequence) (Cysteine residue).
Database link: P04179 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab118340 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab118340の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.01 - 0.1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 25 kDa).
A 1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.01 - 0.1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 25 kDa). A 1 hour primary incubation is recommended for this product. |
ターゲット情報
-
機能
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. -
関連疾患
Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
配列類似性
Belongs to the iron/manganese superoxide dismutase family. -
翻訳後修飾
Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
-
参照データベース
- Entrez Gene: 6648 Human
- Entrez Gene: 20656 Mouse
- Entrez Gene: 24787 Rat
- Entrez Gene: 335799 Zebrafish
- Omim: 147460 Human
- SwissProt: P04179 Human
- SwissProt: P09671 Mouse
- SwissProt: P07895 Rat
see all -
別名
- Indophenoloxidase B antibody
- IPO B antibody
- IPOB antibody
see all
画像
-
Western blot - Anti-SOD2/MnSOD antibody (ab118340)All lanes : Anti-SOD2/MnSOD antibody (ab118340)
Lane 1 : Human Cerebellum lysate
Lane 2 : Mouse Brain lysate
Lane 3 : Rat Brain lysate
Lane 4 : Pig Brain lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 25 kDa -
Western blot - Anti-SOD2/MnSOD antibody (ab118340)All lanes : Anti-SOD2/MnSOD antibody (ab118340)
Lane 1 : HeLa cell lysate
Lane 2 : HepG2
Lane 3 : HEK293
Lane 4 : NIH3T3
Lysates/proteins at 35 µg per lane.
Predicted band size: 25 kDa -
Western blot - Anti-SOD2/MnSOD antibody (ab118340)Anti-SOD2/MnSOD antibody (ab118340) at 0.1 µg/ml (incubation time: 1 hour) + Mouse Spinal Cord lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 25 kDa
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab118340 は 2 報の論文で使用されています。
- Cai X et al. lncRNA FGD5 antisense RNA 1 upregulates RORA to suppress hypoxic injury of human cardiomyocyte cells by inhibiting oxidative stress and apoptosis via miR-195. Mol Med Rep 22:4579-4588 (2020). PubMed: 33174051
- Huang Y et al. Biological effects of pyrroloquinoline quinone on liver damage in Bmi-1 knockout mice. Exp Ther Med 10:451-458 (2015). PubMed: 26622336