Anti-SOCS3 抗体 [SO1] (ab14939)
![Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody [SO1] (ab14939)](https://www.abcam.co.jp/ps/products/14/ab14939/Images/ab14939-22206-anti-socs3-antibody-so1-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody [SO1] (ab14939)")
Key features and details
- Mouse monoclonal [SO1] to SOCS3
- Suitable for: Flow Cyt, ELISA, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-SOCS3 antibody [SO1]
SOCS3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [SO1] to SOCS3 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, ELISA, WB, ICC/IFmore details -
種交差性
交差種: Rat, Human -
免疫原
Full length native protein (purified) (Human).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
SO1 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14939の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| ELISA |
Use at an assay dependent concentration.
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| WB | (2) |
Use at an assay dependent concentration.
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| 特記事項 |
|---|
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Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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ELISA
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST. -
組織特異性
Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus. -
パスウェイ
Protein modification; protein ubiquitination. -
関連疾患
Note=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients. -
配列類似性
Contains 1 SH2 domain.
Contains 1 SOCS box domain. -
ドメイン
The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. -
翻訳後修飾
Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1. - Information by UniProt
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参照データベース
- Entrez Gene: 9021 Human
- Entrez Gene: 89829 Rat
- Omim: 604176 Human
- SwissProt: O14543 Human
- SwissProt: O88583 Rat
- Unigene: 527973 Human
- Unigene: 127801 Rat
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別名
- ATOD4 antibody
- CIS 3 antibody
- CIS-3 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody [SO1] (ab14939)ICC/IF image of ab14939 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14939, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Flow Cytometry - Anti-SOCS3 antibody [SO1] (ab14939)](https://www.abcam.co.jp/ps/products/14/ab14939/Images/ab14939-22205-anti-socs3-antibody-so1-flow-cytometry.jpg)
Flow Cytometry - Anti-SOCS3 antibody [SO1] (ab14939)Overlay histogram showing Jurkat cells stained with ab14939 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14939, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab14939 は 15 報の論文で使用されています。
- Wang XZ et al. Human cytomegalovirus pUL97 upregulates SOCS3 expression via transcription factor RFX7 in neural progenitor cells. PLoS Pathog 19:e1011166 (2023). PubMed: 36753521
- Bermejo-Álvarez I et al. Effects of Tofacitinib on Muscle Remodeling in Experimental Rheumatoid Sarcopenia. Int J Mol Sci 24:N/A (2023). PubMed: 37685986
- Wang R et al. Molecular mechanisms involved in the IL-6-mediated upregulation of indoleamine 2,3-dioxygenase 1 (IDO1) expression in the chorionic villi and decidua of women in early pregnancy. BMC Pregnancy Childbirth 22:983 (2022). PubMed: 36587196
- Li M et al. Axl is related to inflammation in hemodialysis patients. Mol Immunol 133:146-153 (2021). PubMed: 33667984
- Liang E et al. COP9 Signalosome Subunit 3 Restricts Neuroinflammatory Responses During Cerebral Ischemia/Reperfusion Injury Through Stabilizing Suppressor of Cytokine Signaling 3 Protein. Neuropsychiatr Dis Treat 17:1217-1227 (2021). PubMed: 33911869