AB14939

Anti-SOCS3 抗体 [SO1]

Name: Anti-SOCS3 抗体 [SO1] (ab14939) | Abcam
Brand: Abcam Limited
SKU: AB14939
Price: 78000 JPY
Availability: InStock
Rating: 4 (4 reviews)

Anti-SOCS3 antibody [SO1]

(4 Reviews)

(19 Publications)

Mouse Monoclonal SOCS3 antibody. Suitable for Flow Cyt, ELISA, WB, ICC/IF and reacts with Human, Rat samples. Cited in 19 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human SOCS3.

別名を表示する

CIS3, SSI3, SOCS3, Suppressor of cytokine signaling 3, SOCS-3, Cytokine-inducible SH2 protein 3, STAT-induced STAT inhibitor 3, CIS-3, SSI-3

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody [SO1] (AB14939)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody [SO1] (AB14939)

ICC/IF image of ab14939 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14939, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Flow Cytometry - Anti-SOCS3 antibody [SO1] (AB14939)

Flow Cyt

Unknown

Flow Cytometry - Anti-SOCS3 antibody [SO1] (AB14939)

Overlay histogram showing Jurkat cells stained with ab14939 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14939, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

SO1

アイソタイプ

IgG2b

キャリアフリー

交差種

Human, Rat

アプリケーション

ELISA, Flow Cyt, WB, ICC/IF

applications

免疫原

Native Full Length Protein corresponding to Human SOCS3.

O14543

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including IL6ST/gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity and regulates IL6 signaling. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 15601820).

See full target information SOCS3

style

left-column

文献 (19)

Recent publications for all applications. Explore the full list and refine your search

Journal of periodontal & implant science 55:255-272 PubMed40047181

2025

Conditioned medium from cultured cementoblasts upregulates amelotin gene expression via the SOCS3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yohei Nakayama,Kazuma Igarashi,Zhenyu Jin,Arisa Yamaguchi,Bernhard Ganss,Yorimasa Ogata

Emerging microbes & infections 13:2366359 PubMed38855910

2024

Deciphering lung granulomas in HIV & TB co-infection: unveiling macrophages aggregation with IL6R/STAT3 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Li,Cheng Wang,Jizhou Gou,Simo Kitanovski,XiangYi Tang,Yixuan Cai,Chenxia Zhang,Xiling Zhang,Zhenfeng Zhang,Yuanwang Qiu,Fang Zhao,Mengji Lu,Yun He,Jun Wang,Hongzhou Lu

International journal of molecular sciences 24: PubMed37685986

2023

Effects of Tofacitinib on Muscle Remodeling in Experimental Rheumatoid Sarcopenia.

Applications

Unspecified application

Species

Unspecified reactive species

Ismael Bermejo-Álvarez,Sandra Pérez-Baos,Paula Gratal,Juan Pablo Medina,Raquel Largo,Gabriel Herrero-Beaumont,Aránzazu Mediero

PLoS pathogens 19:e1011166 PubMed36753521

2023

Human cytomegalovirus pUL97 upregulates SOCS3 expression via transcription factor RFX7 in neural progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xian-Zhang Wang,Le Wen,Yue-Peng Zhou,Sheng-Nan Huang,Bo Yang,Shuang Cheng,Wen-Bo Zeng,Meng-Jie Mei,Jin-Yan Sun,Xuan Jiang,Han Cheng,Min-Hua Luo

BMC pregnancy and childbirth 22:983 PubMed36587196

2022

Molecular mechanisms involved in the IL-6-mediated upregulation of indoleamine 2,3-dioxygenase 1 (IDO1) expression in the chorionic villi and decidua of women in early pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Wang,Shuyun Zhao,Xiaojuan Chen,Ziwen Xiao,Xinghui Wen,Xingming Zhong,Shixiang Li,Hui Cheng,Guanyou Huang

CNS neuroscience & therapeutics 28:1393-1408 PubMed35702948

2022

Unraveling the role of Epac1-SOCS3 signaling in the development of neonatal-CRD-induced visceral hypersensitivity in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Si-Ting Huang,Bin-Bin Chen,Zhi-Jing Song,Hui-Li Tang,Rong Hua,Yong-Mei Zhang

Current issues in molecular biology 44:1087-1114 PubMed35723295

2022

Renoprotective Effect of Liraglutide Is Mediated via the Inhibition of TGF-Beta 1 in an LLC-PK1 Cell Model of Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Vjera Ninčević,Milorad Zjalić,Tea Omanović Kolarić,Martina Smolić,Tomislav Kizivat,Lucija Kuna,Aleksandar Včev,Ashraf Tabll,Ines Bilić Ćurčić

Neuropsychiatric disease and treatment 17:1217-1227 PubMed33911869

2021

COP9 Signalosome Subunit 3 Restricts Neuroinflammatory Responses During Cerebral Ischemia/Reperfusion Injury Through Stabilizing Suppressor of Cytokine Signaling 3 Protein.

Applications

Unspecified application

Species

Unspecified reactive species

En Liang,Xiaojun Li,Wenjun Fu,Changtong Zhao,Baoying Yang,Zhonghua Yang

Molecular immunology 133:146-153 PubMed33667984

2021

Axl is related to inflammation in hemodialysis patients.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyuan Li,Wen Xue,Xinhua Li,Yaxiang Song,Xinying Liu,Ling Qin

American journal of translational research 12:7287-7296 PubMed33312367

2020

Effects of miR-22-3p targeted regulation of Socs3 on the hepatic insulin resistance in mice with gestational diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Zhang,Qiaoyi Wang,Kun Yang,Hanjing Zheng,Yujing Hu

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SOCS3 抗体 [SO1]

Anti-SOCS3 antibody [SO1]

Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody [SO1] (AB14939)

Flow Cytometry - Anti-SOCS3 antibody [SO1] (AB14939)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (19)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise