Anti-SOCS3 抗体 (ab16030)
Key features and details
- Rabbit polyclonal to SOCS3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SOCS3 antibody
SOCS3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SOCS3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Cow, Dog -
免疫原
Synthetic peptide corresponding to Human SOCS3 aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab16199) -
ポジティブ・コントロール
- WB: KARPAS-299 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16030の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (9) |
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
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ICC/IF | (2) |
Use a concentration of 1 µg/ml.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa. |
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST. -
組織特異性
Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus. -
パスウェイ
Protein modification; protein ubiquitination. -
関連疾患
Note=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients. -
配列類似性
Contains 1 SH2 domain.
Contains 1 SOCS box domain. -
ドメイン
The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. -
翻訳後修飾
Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1. - Information by UniProt
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参照データベース
- Entrez Gene: 395299 Chicken
- Entrez Gene: 282081 Cow
- Entrez Gene: 442949 Dog
- Entrez Gene: 9021 Human
- Entrez Gene: 12702 Mouse
- Entrez Gene: 89829 Rat
- Omim: 604176 Human
- SwissProt: Q90X67 Chicken
see all -
別名
- ATOD4 antibody
- CIS 3 antibody
- CIS-3 antibody
see all
画像
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Lanes 1-3 : Anti-SOCS3 antibody [EPR24090-74] (ab280884) at 1/1000 dilution
Lanes 4-6 : Anti-SOCS3 antibody (ab16030) at 1/1000 dilution
Lanes 1 & 4 : KARPAS-299 (Human anaplastic large cell lymphoma) whole cell lysate
Lanes 2 & 5 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lanes 3 & 6 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 23,26 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and dilution buffer: 5% NFDM/TBST.
ab181602 was used as loading control at 1/1000000 dilution.
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All lanes : Anti-SOCS3 antibody (ab16030) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SOCS3 peptide (ab16199) at 1 µg/ml
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human SOCS3 peptide (ab16199) at 1 µg/ml
Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SOCS3 peptide (ab16199) at 1 µg/ml
Lane 8 : MOLT-4 (Human) Whole Cell Lysate (ab52252) with Human SOCS3 peptide (ab16199) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
The 30kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to SOCS3. -
ICC/IF image of ab16030 stained human HeLa cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab16030, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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All lanes : Anti-SOCS3 antibody (ab16030) at 1 µg/ml
Lane 1 : Untreated Human peripheral blood mononuclear cell lysate
Lane 2 : Human peripheral blood mononuclear cell lysate (treated with aCD3/28)
Lane 3 : Human peripheral blood mononuclear cell lysate (treated with IFNγ)
Lane 4 : SOCS3 protein
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit polyclonal IgG
at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 15 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (134)
ab16030 は 134 報の論文で使用されています。
- Rubio T et al. Beneficial Effect of Fingolimod in a Lafora Disease Mouse Model by Preventing Reactive Astrogliosis-Derived Neuroinflammation and Brain Infiltration of T-lymphocytes. Mol Neurobiol 61:3105-3120 (2024). PubMed: 37971656
- Qian C et al. Opening KATP channels induces inflammatory tolerance and prevents chronic pain. Brain Behav Immun 107:76-86 (2023). PubMed: 36198341
- Liu B et al. PTK6 inhibits autophagy to promote uveal melanoma tumorigenesis by binding to SOCS3 and regulating mTOR phosphorylation. Cell Death Dis 14:55 (2023). PubMed: 36690663
- Wang XZ et al. Human cytomegalovirus pUL97 upregulates SOCS3 expression via transcription factor RFX7 in neural progenitor cells. PLoS Pathog 19:e1011166 (2023). PubMed: 36753521
- Li X et al. SOCS3 as a potential driver of lung metastasis in colon cancer patients. Front Immunol 14:1088542 (2023). PubMed: 37025997