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Anti-SOCS1 抗体 - Carboxyterminal end (ab65989)

Submit a review Q&A (1)References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS1 antibody - Carboxyterminal end (ab65989)
  • Western blot - Anti-SOCS1 antibody - Carboxyterminal end (ab65989)

Key features and details

  • Rabbit polyclonal to SOCS1 - Carboxyterminal end
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-SOCS1 antibody - Carboxyterminal end
    SOCS1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to SOCS1 - Carboxyterminal end

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Rat, Human

  • 免疫原

    A synthetic peptide corresponding to a sequence at the C-terminal of human SOCS1, identical to the related mouse and rat sequence.

  • ポジティブ･コントロール

    • Rat lung tissue.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab65989の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 24 kDa.
IHC-P
Use a concentration of 0.5 - 1 µg/ml.
特記事項
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 24 kDa.
IHC-P
Use a concentration of 0.5 - 1 µg/ml.

ターゲット情報

  • 機能

    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2.

  • 組織特異性

    Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.

  • パスウェイ

    Protein modification; protein ubiquitination.

  • 配列類似性

    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.

  • ドメイン

    The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CIS1 antibody
      • CISH 1 antibody
      • CISH1 antibody
      • Cytokine inducible SH2 protein 1 antibody
      • JAB antibody
      • JAK binding protein antibody
      • JAK-binding protein antibody
      • Janus kinase binding protein antibody
      • SOCS 1 antibody
      • SOCS-1 antibody
      • Socs1 antibody
      • SOCS1_HUMAN antibody
      • SSI 1 antibody
      • SSI-1 antibody
      • SSI1 antibody
      • STAT induced STAT inhibitor 1 antibody
      • STAT-induced STAT inhibitor 1 antibody
      • Suppressor of cytokine signaling 1 antibody
      • Supressor of cytokine signalling 1 antibody
      • TEC interacting protein 3 antibody
      • Tec-interacting protein 3 antibody
      • TIP 3 antibody
      • TIP-3 antibody
      • TIP3 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS1 antibody - Carboxyterminal end (ab65989)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS1 antibody - Carboxyterminal end (ab65989)
      ab65989, at 0.5µg/ml, staining SOCS1 in paraffin-embedded rat lung tissue by immunohistochemistry. Staining of SOCS1 in the nucleus is observed. DAB chromogenic reaction.
    • Western blot - Anti-SOCS1 antibody - Carboxyterminal end (ab65989)
      Western blot - Anti-SOCS1 antibody - Carboxyterminal end (ab65989)
      All lanes : Anti-SOCS1 antibody - Carboxyterminal end (ab65989)

      Lane 1 : HT1080 Cell Lysate
      Lane 2 : COLO320 Cell Lysate

      Predicted band size: 24 kDa

    参考文献 (1)

    ab65989 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab65989 は 1 報の論文で使用されています。

    • Muratsu D  et al. Zoledronic acid enhances lipopolysaccharide-stimulated proinflammatory reactions through controlled expression of SOCS1 in macrophages. PLoS One 8:e67906 (2013). WB . PubMed: 23874464

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Product code: 3691
    Inquiry: Hi I am looking for the antibodies which could be used for human SOCS1 detection by FACS and IHC. I see that you have the facs antibody for SOCS1, could you please send me the protocol and also the optimized concentration? Thank you very much! And also I saw you have a lot of the antibodies for IHC staining for SOCS1. I would like to check the human SOCS1 expression in different organs of humanized mice. Could you give a recommendation? Thank you very much! Best regards

    Read More
    Answer

    Thank you for contacting us.

    To our knowledge, customised protocols are not required for this product, any general Flow Cytometry protocol could be used with this antibody.

    https://www.abcam.com/index.html?pageconfig=resource&rid=11380

    https://www.abcam.com/index.html?pageconfig=resource&rid=11448

    https://www.abcam.com/index.html?pageconfig=resource&rid=11381

    https://www.abcam.com/index.html?pageconfig=popular_protocols

    The optimal dilutions should be determined by end user.I wouldsuggest starting anywhere from1ug-10ug/ml.

    All of these antibodies are tested with human samples and in IHC-P e.g. ab62584, ab9870, ab135718, ab83493, ab65989 so these would work. Choose any one of these for best results. Please note,because you are using mouse for tissue grafts so avoiding mouse monoclonal would be a good idea else we can suggest some extra blocking steps.

    The concentration of antibody for IHC-P can be tried somewhere between 1-10ug/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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