Anti-SOCS1 抗体 (ab9870)

Thank you for contacting us.

To our knowledge, customised protocols are not required for this product, any general Flow Cytometry protocol could be used with this antibody.

https://www.abcam.com/index.html?pageconfig=resource&rid=11380

https://www.abcam.com/index.html?pageconfig=resource&rid=11448

https://www.abcam.com/index.html?pageconfig=resource&rid=11381

https://www.abcam.com/index.html?pageconfig=popular_protocols

The optimal dilutions should be determined by end user.I wouldsuggest starting anywhere from1ug-10ug/ml.

All of these antibodies are tested with human samples and in IHC-P e.g. ab62584, ab9870, ab135718, ab83493, ab65989 so these would work. Choose any one of these for best results. Please note,because you are using mouse for tissue grafts so avoiding mouse monoclonal would be a good idea else we can suggest some extra blocking steps.

The concentration of antibody for IHC-P can be tried somewhere between 1-10ug/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for your enquiry. The differences between goat polyclonal to SOCS1 (ab9870) and rabbit polyclonal to SOCS1 (ab3691) is that ab9870 reacts with mouse and is predicted to react with human due to sequence homology. So far it has only been shown to work by western blotting. ab3691 reacts with human and has been shown to work by western blotting, immunoprecipitation and ELISA. We do not have details as to the best one of the two for application to human melanoma cells. Yes, you will need an anti-goat IgG secondary antibody for use with SOCS1 antibody (ab9870). For a detailed list of compatible secondaries may I suggest that you visit the datasheet of the antibody and scroll to the bottom. Unfortunately we do not sell any antibodies that are specific for the phosphorylation status of STAT2. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your patience. As ab5923 was originated outside Abcam I had to contact the originator regarding your question and have just heard back. There was a mistake on the datasheet for ab5923 - it is a CIS1 antibody, not a SOCS1 antibody. I apologize for this mistake and the datasheet will be updated to reflect this information. I can of course offer you a refund or an alternative antibody free of charge. Ab9870 is a SOCS1 antibody, as well as ab3691. Please let me know what you would like.

I'm sorry to hear that you are having problems with these two antibodies. I am enquiring with the originators of these two antibodies for suggestions that will help you out. In the meantime, below is a general protocol for ab9870 that will be helpful for you. Some suggestions: high background is usually a sign of a blocking problem. I know that you mentioned that you have tried different blocks, but the secondary may be reacting with the blocking reagent. I suggest that you test this by omiting the primary antibody and testing with only the secondary. If background appears, change either the secondary or the blocking reagent. Also, please try using the secondary at a lower concnetration. Protocol for ab9870: After transfer on PVDF membrane, membrane is blocked in 2.5% skimmed milk in TBS-T for 1 hr at room temperature with agitation (can be blocked overnight at 4C without agitation). We find enhancement of transfer of proteins on PVDF membranes compared to nitrocellulose membranes. Primary antibody (~2ug/ml) was incubated for 1 hr at room temperature with agitation. Then membrane was incubated with the secondary antibody for 1 hr at room temperature with agitation. Washing with TBST three times after primary and secondary antibody, each wash is about for 5-10 mins. We use ECL-plus (Amersham) rather than ECL, which is more sensitive for detection.