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AB326284

SNv428 Anti-Visfatin antibody [EPR21980]

SNv428 Anti-Visfatin antibody [EPR21980]

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Rabbit Recombinant Monoclonal Visfatin antibody - conjugated to SNv428. Suitable for Flow Cyt(Intra) and reacts with Human samples.

This is a Beckman Coulter product distributed by Abcam.

別名を表示する

PBEF, PBEF1, NAMPT, Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1, Visfatin, Pre-B cell-enhancing factor

1 Images
Flow Cytometry (Intracellular) - SNv428 Anti-Visfatin antibody [EPR21980] (AB326284)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - SNv428 Anti-Visfatin antibody [EPR21980] (AB326284)

Flow cytometry scatter plots showing human peripheral mononuclear cells (PBMCs) stained with ab326284 (right) or FMO control (left). The cells were fixed and permeabilised using BD CytoFix/CytoPerm™ (20 min). The cells were incubated in 1x PBS containing 10 μg/ml human IgG, 10% normal goat serum and BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer (1/10) to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab326284) (1x 106 100μl at 5 μg/ml (1/40)) for 30min on ice. The cells were simultaneously stained with CD3.

Acquisition of >30000 events were collected using a 100 mW Violet laser (405nm) and 450/45 bandpass filter.

This data was acquired using a Beckman Coulter CytoFlexLX flow cytometer.

関連する標識済み抗体及び組成の異なる製品 (6)

  • Unconjugated

    Anti-Visfatin antibody [EPR21980]

  • Carrier free

    Anti-Visfatin antibody [EPR21980] - BSA and Azide free (Capture)

  • Carrier free

    Anti-Visfatin antibody [EPR21980] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Visfatin antibody [EPR21980]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980]

  • 578 PE

    PE Anti-Visfatin antibody [EPR21980]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR21980

アイソタイプ

IgG

標識

SNv428

励起波長/蛍光波長

Ex: 405nm, Em: 428nm

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/40", "FlowCytIntra-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This is a Beckman Coulter product distributed by Abcam.

The SuperNova dye included in this product is provided under an intellectual property license from Beckman Coulter Life Sciences. As this product contains the SuperNova dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity).

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA, 0.02% Pluronic F-68
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
+4°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component : CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).
See full target information NAMPT
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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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