SNv428 Anti-Transketolase antibody [7H1AA1]

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - SNv428 Anti-Transketolase antibody [7H1AA1] (AB326277)

Flow cytometry scatter plots showing human peripheral mononuclear cells (PBMCs) stained with ab326277 (right) or FMO control (left). The cells were fixed and permeabilised using BD CytoFix/CytoPerm™ (20 min). The cells were incubated in 1x PBS containing 10 μg/ml human IgG, 10% normal goat serum and BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer (1/10) to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab326277) (1x 10⁶ 100μl at 0.2 μg/ml (1/1000)) for 30min on ice. The cells were simultaneously stained with CD3.

Acquisition of >30000 events were collected using a 100 mW Violet laser (405nm) and 450/45 bandpass filter.

This data was acquired using a Beckman Coulter CytoFlexLX flow cytometer.