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AB326142

SNv428 Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668]

SNv428 Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668]

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Rabbit Recombinant Monoclonal Glucose 6 Phosphate Dehydrogenase antibody - conjugated to SNv428. Suitable for Flow Cyt (Intra) and reacts with Human samples.

This is a Beckman Coulter product distributed by Abcam

別名を表示する

Glucose-6-phosphate 1-dehydrogenase, G6PD

1 Images
Flow Cytometry (Intracellular) - SNv428 Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB326142)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - SNv428 Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB326142)

Flow cytometry scatter plots showing untreated human peripheral mononuclear cells (PBMCs) (top) and PBMCs activated with 5 µg/ml PHA for 18h (bottom), either stained with ab326142 (right) or FMO control (left). The cells were fixed and permeabilised using BD CytoFix/CytoPerm™ (20 min). The cells were incubated in 1x PBS containing 10 µg/ml human IgG, 10% normal goat serum and BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer (1/10) to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab326142) (1x 106 in 100µl at 1 µg/ml (1/200)) for 30min on ice. The cells were simultaneously stained with CD3. Acquisition of >30000 events were collected using a 100 mW Violet laser (405nm) and 450/45 bandpass filter.This data was acquired using a Beckman Coulter CytoFlexLX flow cytometer.

関連する標識済み抗体及び組成の異なる製品 (5)

  • Unconjugated

    Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668]

  • Mouse version

    Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - Mouse IgG1 (Chimeric)

  • Mouse version

    Anti-G6PD antibody [EPR20668] - Mouse IgG1 (Chimeric) - BSA and Azide free

  • Carrier free

    Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free

  • 578 PE

    PE Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20668

アイソタイプ

IgG

標識

SNv428

励起波長/蛍光波長

Ex: 405nm, Em: 428nm

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "<p>Use of the SuperNova Staining Buffer (<a href='/products/buffers/supernova-staining-buffer-ab327075'>ab327075</a>) is recommended when your multicolor staining cocktail contains more than one SuperNova/polymer dye antibody conjugate.</p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This is a Beckman Coulter product distributed by Abcam.

The SuperNova dye included in this product is provided under an intellectual property license from Beckman Coulter Life Sciences. As this product contains the SuperNova dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity).

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA, 0.02% Pluronic F-68
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
+4°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Glucose 6 Phosphate Dehydrogenase also known as G6PD or G6P dehydrogenase plays an important role in the pentose phosphate pathway catalyzing the conversion of glucose 6-phosphate to 6-phosphoglucono-Δ-lactone while producing NADPH from NADP+. G6PD with a molecular mass of approximately 59 kDa is expressed in various tissues with high levels found in red blood cells liver and adrenal glands. This enzyme is vital for protecting cells from oxidative damage by supplying reductive capacity.
Biological function summary

Glucose 6-phosphate dehydrogenase is essential for maintaining cellular redox balance especially in cells lacking mitochondria like red blood cells. It functions as part of a monomer which can dimerize depending on the cellular needs and conditions. The activity of G6PD directly affects the production of NADPH which is necessary for the biosynthesis of nucleic acids and lipids and for maintaining reduced glutathione levels therefore sustaining the antioxidant capacity of the cell.

Pathways

Glucose 6-phosphate dehydrogenase is a pivotal enzyme in the oxidative phase of the pentose phosphate pathway which provides ribose 5-phosphate for nucleotide synthesis and NADPH for reductive biosynthetic reactions. G6PD connects with other enzymes in the pathway such as 6-phosphogluconate dehydrogenase contributing to the regulation of cellular metabolic needs. Its function is tightly linked to glucose metabolism and indirectly influences glycolytic processes.

Alterations in glucose 6-phosphate dehydrogenase activity lead to conditions such as G6PD deficiency which can cause hemolytic anemia when individuals are exposed to certain drugs infections or foods. This enzyme's deficiency is one of the most common enzymatic disorders in humans and is triggered by genetic mutations affecting G6PD function. Moreover G6PD has implications in cancer biology as its activity influences the proliferation and survival of cancer cells alongside other proteins like TP53 and NF-κB which are involved in cellular stress responses.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
See full target information Glucose-6-phosphate 1-dehydrogenase
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Abcam product promise

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