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AB326140

SNv428 Anti-GAPDH antibody [EPR16891]

SNv428 Anti-GAPDH antibody [EPR16891]

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Rabbit Recombinant Monoclonal GAPDH antibody - conjugated to SNv428. Suitable for Flow Cyt (Intra) and reacts with Human samples.

This is a Beckman Coulter product distributed by Abcam

別名を表示する

GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

1 Images
Flow Cytometry (Intracellular) - SNv428 Anti-GAPDH antibody [EPR16891] (AB326140)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - SNv428 Anti-GAPDH antibody [EPR16891] (AB326140)

Flow cytometry scatter plots showing untreated human peripheral mononuclear cells (PBMCs) (top) and PBMCs activated with 5 µg/ml PHA for 18h (bottom), either stained with ab325140 (right) or FMO control (left). The cells were fixed and permeabilised using BD CytoFix/CytoPerm™ (20 min). The cells were incubated in 1x PBS containing 10 µg/ml human IgG, 10% normal goat serum and BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer (1/10) to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab326140) (1x 106 in 100µl at 1 µg/ml (1/200)) for 30min on ice. The cells were simultaneously stained with CD3. Acquisition of >30000 events were collected using a 100 mW Violet laser (405nm) and 450/45 bandpass filter.This data was acquired using a Beckman Coulter CytoFlexLX flow cytometer.

関連する標識済み抗体及び組成の異なる製品 (9)

  • Unconjugated

    Anti-GAPDH antibody [EPR16891] - Loading Control

  • Carrier free

    Anti-GAPDH antibody [EPR16891] - BSA and Azide free

  • 421 Alexa Fluor® 405

    Alexa Fluor® 405 Anti-GAPDH antibody [EPR16891]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-GAPDH antibody [EPR16891]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-GAPDH antibody [EPR16891]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-GAPDH antibody [EPR16891]

  • 519 FITC

    FITC Anti-GAPDH antibody [EPR16891]

  • HRP

    HRP Anti-GAPDH antibody [EPR16891] - Loading Control

  • 578 PE

    PE Anti-GAPDH antibody [EPR16891]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR16891

アイソタイプ

IgG

標識

SNv428

励起波長/蛍光波長

Ex: 405nm, Em: 428nm

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "<p>Use of the SuperNova Staining Buffer (<a href='/products/buffers/supernova-staining-buffer-ab327075'>ab327075</a>) is recommended when your multicolor staining cocktail contains more than one SuperNova/polymer dye antibody conjugate.</p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This is a Beckman Coulter product distributed by Abcam.

The SuperNova dye included in this product is provided under an intellectual property license from Beckman Coulter Life Sciences. As this product contains the SuperNova dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity).

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA, 0.02% Pluronic F-68
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
+4°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.
Biological function summary

GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.

Pathways

GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.

GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH's altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis (PubMed : 11724794, PubMed : 3170585). Also shows nitrosylase activity, thereby playing a role in nuclear functions (PubMed : 11724794, PubMed : 3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed : 23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed : 23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed : 23332158, PubMed : 27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
See full target information GAPDH
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Abcam product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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