AB154870

Anti-SNRPF 抗体 [EPR10388]

Anti-SNRPF antibody [EPR10388]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal SNRPF antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

別名を表示する

PBSCF, SNRPF, Small nuclear ribonucleoprotein F, snRNP-F, Sm protein F, Sm-F, SmF

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] (AB154870)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] (AB154870)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling SNRPF with ab154870 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] (AB154870)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] (AB154870)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SNRPF with ab154870 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] (AB154870)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] (AB154870)

Immunohistochemical analysis of paraffin embedded Human normal pancreas tissue using ab154870 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] (AB154870)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] (AB154870)

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab154870 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] (AB154870)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] (AB154870)

Immunohistochemical analysis of paraffin embedded Human normal prostate tissue using ab154870 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] (AB154870)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] (AB154870)

Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154870 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-SNRPF antibody [EPR10388] (AB154870)

WB

Unknown

Western blot - Anti-SNRPF antibody [EPR10388] (AB154870)

All lanes:

Western blot - Anti-SNRPF antibody [EPR10388] (ab154870) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

MCF-7 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

HL-60 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 10 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

Carrier free

Anti-SNRPF antibody [EPR10388] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR10388

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, WB

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SNRPF also known as Small Nuclear Ribonucleoprotein Polypeptide F plays a mechanical role in the spliceosomal complex. It is a component of the U1 U2 U4 U5 and U6 small nuclear ribonucleoproteins (snRNPs) essential in mRNA splicing. This protein has a mass of approximately 9.5 kDa. It is mainly expressed in the nucleus of eukaryotic cells where it participates in RNA processing.

Biological function summary

SNRPF functions as a part of the spliceosome assembly a large ribonucleoprotein complex responsible for pre-mRNA splicing. This process modifies nascent RNA transcripts converting them into mature mRNA for translation. The role of SNRPF in association with other Sm core proteins enables the assembly and stabilization of the snRNPs facilitating the accurate execution of the splicing cycle.

Pathways

The involvement of SNRPF in mRNA processing stands out significantly. It acts within the spliceosome complex playing an important part in the mRNA splicing pathway. This pathway ensures the removal of introns and the proper joining of exons vital for producing functional RNA transcripts. SNRPF interacts with other proteins like SNRPD1 and SNRPD2 forming an intricate network pivotal in the regulation of gene expression.

Defects in SNRPF expression or function have links to neurodegenerative disorders and certain cancers. Misregulation of splicing events potentially caused by altered levels of SNRPF connects to conditions such as spinal muscular atrophy. The protein's interactions with other splicing factors might influence the development of these diseases highlighting the importance of its proper regulation in cellular processes.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed : 11991638, PubMed : 18984161, PubMed : 19325628, PubMed : 23333303, PubMed : 25555158, PubMed : 26912367, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166, PubMed : 32494006). Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes (PubMed : 11991638, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077). As part of the U7 snRNP it is involved in histone 3'-end processing (PubMed : 12975319).

See full target information SNRPF

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 15:4623-4635 PubMed39006089

2024

Apolipoprotein L3 inhibits breast cancer proliferation and modulates cell cycle via the P53 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Yu,Siyan Li,Xing Li,Yanbiao Liu,Zhaobu Wang,Mengyao Cui,Feng Jin,Xinmiao Yu

View all publications

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Abcam product promise

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