Anti-SNF2L 抗体 [EPR12190] (ab175226)
![Immunoprecipitation - Anti-SNF2L antibody [EPR12190] (ab175226)](https://www.abcam.co.jp/ps/products/175/ab175226/Images/ab175226-202805-anti-snf2l-antibody-epr12190-immunoprecipitation.jpg "Immunoprecipitation - Anti-SNF2L antibody [EPR12190] (ab175226)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12190] to SNF2L
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SNF2L antibody [EPR12190]
SNF2L 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12190] to SNF2L -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, IPmore details
適用なし: Flow Cyt -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Synthetic peptide within Human SNF2L aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P28370 -
ポジティブ･コントロール
- Hela, 293T and SH-SY5Y cell lysates; Human stomach tissue; 293T cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12190 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175226の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/250.
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| IP |
1/10 - 1/100.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 123 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
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IP
1/10 - 1/100. |
ターゲット情報
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関連性
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. Two transcript variants encoding different isoforms have been found for this gene. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 6594 Human
- Omim: 300012 Human
- SwissProt: P28370 Human
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別名
- ATP dependent helicase SMARCA1 antibody
- global transcription activator homologous sequence antibody
- ISWI antibody
see all
画像
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Immunoprecipitation - Anti-SNF2L antibody [EPR12190] (ab175226)
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab175226.
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![Immunocytochemistry/ Immunofluorescence - Anti-SNF2L antibody [EPR12190] (ab175226)](https://www.abcam.co.jp/ps/products/175/ab175226/Images/ab175226-201666-anti-snf2l-antibody-epr12190-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SNF2L antibody [EPR12190] (ab175226)Immunofluorescent analysis of 293T cells labeling SNF2L with ab175226 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF2L antibody [EPR12190] (ab175226)](https://www.abcam.co.jp/ps/products/175/ab175226/Images/ab175226-201665-anti-snf2l-antibody-epr12190-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF2L antibody [EPR12190] (ab175226)Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling SNF2L with ab175226 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-SNF2L antibody [EPR12190] (ab175226)](https://www.abcam.co.jp/ps/products/175/ab175226/Images/ab175226-201664-anti-snf2l-antibody-epr12190-western-blot.jpg)
Western blot - Anti-SNF2L antibody [EPR12190] (ab175226)All lanes : Anti-SNF2L antibody [EPR12190] (ab175226) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 123 kDa -
![Anti-SNF2L antibody [EPR12190] (ab175226)](https://www.abcam.co.jp/ps/products/175/ab175226/Images/ab175226-5-benefits-of-recombinant-antibodies.png)
Anti-SNF2L antibody [EPR12190] (ab175226)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab175226 は論文での使用が確認できていません。