Anti-SNF2H 抗体 - ChIP Grade (ab3749)
Key features and details
- Rabbit polyclonal to SNF2H - ChIP Grade
- Suitable for: IP, ICC/IF, ChIP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SNF2H antibody - ChIP Grade
SNF2H 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SNF2H - ChIP Grade -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab183730.
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アプリケーション
適用あり: IP, ICC/IF, ChIP, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3749の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | (1) |
Use at an assay dependent concentration. PubMed: 16917504
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| ICC/IF | (3) |
Use at an assay dependent concentration.
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| ChIP | (1) |
Use 2.5µl for 106 cells.
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| WB | (4) |
1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 122 kDa).
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| 特記事項 |
|---|
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IP
Use at an assay dependent concentration. PubMed: 16917504 |
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ICC/IF
Use at an assay dependent concentration. |
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ChIP
Use 2.5µl for 106 cells. |
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WB
1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 122 kDa). |
ターゲット情報
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機能
Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 SANT domains. -
発生段階
Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 8467 Human
- Entrez Gene: 93762 Mouse
- Omim: 603375 Human
- SwissProt: O60264 Human
- SwissProt: Q91ZW3 Mouse
- Unigene: 558422 Human
- Unigene: 246803 Mouse
- Unigene: 474173 Mouse
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別名
- 4933427E24Rik antibody
- D030040M08Rik antibody
- D330027N15Rik antibody
see all
画像
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Western blot - Anti-SNF2H antibody - ChIP Grade (ab3749)
Rabbit polyclonal ab3749 to SNF2H was used in all lanes at 1/500 dilution.
Lane 1: HeLa lysate (20ug)
Lane 2: Mouse 3T3 lysate (20ug)
Lane 3: HeLa lysate (20ug), blocking peptide (ab5160) 1.0ug
Lane 4: Mouse 3T3 lysate (20ug), blocking peptide (ab5160) 1.0ug
Secondary antibody: Goat polyclonal to Rabbit IgG (ab6721)ab3749 was able to recognise SNF2H in HeLA and Mouse 3T3 lysates (band at ~120 kDa). Binding was blocked by the immunising peptide ab5160.
Rabbit polyclonal ab3749 to SNF2H was used in all lanes at 1/500 dilution.
Lane 1: HeLa lysate (20ug)
Lane 2: Mouse 3T3 lysate (20ug)
Lane 3: HeLa lysate (20ug), blocking peptide (ab5160) 1.0ug
Lane 4: Mouse 3T3 lysate (20ug), blocking peptide (ab5160) 1.0ug
Secondary antibody: Goat polyclonal to Rabbit IgG (ab6721)
ab3749 was able to recognise SNF2H in HeLA and Mouse 3T3 lysates (band at ~120 kDa). Binding was blocked by the immun -
ChIP - Anti-SNF2H antibody - ChIP Grade (ab3749)ab3749 works in ChIP, as shown by the detection of an increase in the recruitment of SNF2H to the estrogen-responsive pS2 promoter.
Sonicated Chromatin prepared from untreated or 17beta-estradiol (E) treated MCF7 cells was subjected to the ChIP procedure with ab3749 antibody specific to SNF2H. The immunoprecipitated chromatin was analysed in the proximal region of the estro
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (43)
ab3749 は 43 報の論文で使用されています。
- Zhong Y et al. Hierarchical regulation of the resting and activated T cell epigenome by major transcription factor families. Nat Immunol 23:122-134 (2022). PubMed: 34937932
- Wang Y et al. LSD1 is required for euchromatic origin firing and replication timing. Signal Transduct Target Ther 7:102 (2022). PubMed: 35414135
- Abeywickrama-Samarakoon N et al. Hepatitis Delta Virus histone mimicry drives the recruitment of chromatin remodelers for viral RNA replication. Nat Commun 11:419 (2020). PubMed: 31964889
- Sabatella M et al. ERCC1-XPF targeting to psoralen-DNA crosslinks depends on XPA and FANCD2. Cell Mol Life Sci N/A:N/A (2019). PubMed: 31392348
- Salvador Moreno N et al. The nuclear structural protein NuMA is a negative regulator of 53BP1 in DNA double-strand break repair. Nucleic Acids Res 47:2703-2715 (2019). PubMed: 30812030