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Anti-SNF2H 抗体 [3.25(2)] (ab33747)

Submit a review Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Anti-SNF2H antibody [3.25(2)] (ab33747)
  • Western blot - Anti-SNF2H antibody [3.25(2)] (ab33747)
  • Flow Cytometry (Intracellular) - Anti-SNF2H antibody [3.25(2)] (ab33747)

Key features and details

  • Mouse monoclonal [3.25(2)] to SNF2H
  • Suitable for: WB, Flow Cyt (Intra), ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-SNF2H antibody [3.25(2)]
    SNF2H 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [3.25(2)] to SNF2H

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, Flow Cyt (Intra), ICC/IFmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Fusion protein. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • This antibody gave a positive signal in the following Human whole cell lysates: HeLa, Jurkat, HepG2, MCF7 This antibody gave a negative signal in the following Mouse whole cell lysate: NIH3T3

  • 特記事項

    We can conjugate this antibody to FITC for you (please see ab150233 for details).

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein G purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    3.25(2)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab33747の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 10 µg/ml. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).
Flow Cyt (Intra)
Use 1µl for 106 cells.
ICC/IF
Use a concentration of 5 µg/ml.
特記事項
WB
Use a concentration of 10 µg/ml. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).
Flow Cyt (Intra)
Use 1µl for 106 cells.
ICC/IF
Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能

    Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing.

  • 組織特異性

    Ubiquitously expressed.

  • 配列類似性

    Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 SANT domains.

  • 発生段階

    Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 4933427E24Rik antibody
      • D030040M08Rik antibody
      • D330027N15Rik antibody
      • EC 3.6.1.- antibody
      • hISWI antibody
      • hSNF2H antibody
      • ISWI antibody
      • MommeD4 antibody
      • SMARCA5 antibody
      • SMCA5_HUMAN antibody
      • Snf2h antibody
      • Sucrose nonfermenting like 5 antibody
      • Sucrose nonfermenting protein 2 homolog antibody
      • Sucrose nonfermenting, yeast, homolog of antibody
      • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 antibody
      • WCRF135 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-SNF2H antibody [3.25(2)] (ab33747)
      Immunocytochemistry/ Immunofluorescence - Anti-SNF2H antibody [3.25(2)] (ab33747)
      ICC/IF image of ab33747 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33747, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10min) HEK293 cells, HepG2 cells at 5ug/ml and in 100% Methanol fixed (5 min) HeLa cells, HEK293 cells, HepG2 cells, and MCF-7 cells at 5µg/ml.
    • Western blot - Anti-SNF2H antibody [3.25(2)] (ab33747)
      Western blot - Anti-SNF2H antibody [3.25(2)] (ab33747)
      All lanes : Anti-SNF2H antibody [3.25(2)] (ab33747) at 10 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
      Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size: 122 kDa
      Observed band size: 122 kDa

    • Flow Cytometry (Intracellular) - Anti-SNF2H antibody [3.25(2)] (ab33747)
      Flow Cytometry (Intracellular) - Anti-SNF2H antibody [3.25(2)] (ab33747)
      Overlay histogram showing HeLa cells stained with ab33747 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33747, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    参考文献 (1)

    ab33747 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab33747 は 1 報の論文で使用されています。

    • Vidi PA  et al. NuMA promotes homologous recombination repair by regulating the accumulation of the ISWI ATPase SNF2h at DNA breaks. Nucleic Acids Res 42:6365-79 (2014). IP . PubMed: 24753406

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