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AB252660

Anti-SNAP25 抗体 [EPR20249-110] - BSA and Azide free (Capture)

Anti-SNAP25 antibody [EPR20249-110] - BSA and Azide free (Capture)

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Anti-SNAP25 antibody [EPR20249-110] - BSA and Azide free (Capture) (ab252660) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting SNAP25 in ELISA. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

SNAP, SNAP25, Synaptosomal-associated protein 25, SNAP-25, Super protein, Synaptosomal-associated 25 kDa protein, SUP

1 Images
Sandwich ELISA - Anti-SNAP25 antibody [EPR20249-110] - BSA and Azide free (Capture) (AB252660)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-SNAP25 antibody [EPR20249-110] - BSA and Azide free (Capture) (AB252660)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab256394).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20249-110

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Assay Kits

AB256394

SNAP25 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - SNAP25 ELISA Kit (AB256394)

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Sample Prep & Detection Kits

AB270705

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20 (AB270705)

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Sample Prep & Detection Kits

AB269889

Europium Conjugation Kit

Dot Blot - Europium Conjugation Kit (AB269889)

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Proteins & Peptides

AB74529

Recombinant Human SNAP25 protein (Tag Free)

Western blot - Recombinant Human SNAP25 protein (Tag Free) (AB74529)

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Primary Antibodies

AB259649

Anti-PDGFR beta antibody [EPR22290-256] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-PDGFR beta antibody [EPR22290-256] - BSA and Azide free (Capture) (AB259649)

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Primary Antibodies

AB259576

Anti-MIP-3 beta/CCL19 antibody [EPR21965-72] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-MIP-3 beta/CCL19 antibody [EPR21965-72] - BSA and Azide free (Detector) (AB259576)

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Primary Antibodies

AB242608

Anti-CCL23 antibody [EPR19763-47] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-CCL23 antibody [EPR19763-47] - BSA and Azide free (Capture) (AB242608)

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  • 0
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Reactivity data

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製品の詳細

ab252660 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human SNAP25. The recommended pair for sandwich ELISA is:
Capture: ab252660, Human SNAP25 Capture Antibody (unconjugated)
Detector: ab252601, Human SNAP25 Detector Antibody (unconjugated)
The reference range value is 62.5 - 4000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SNAP25 also known as Synaptosomal-associated protein 25 plays a critical role in neurotransmitter release through its involvement in the vesicle fusion process. It is part of the SNARE complex and is essential for the docking and fusion of synaptic vesicles with the presynaptic membrane. The protein is approximately 25 kDa in mass and is expressed abundantly in neurons within the central nervous system. Among its various synonyms you may encounter 'protein SNAP' in some literature.
Biological function summary

SNARE proteins mediate the fusion of transport vesicles with their target membranes. SNAP25 as part of this complex facilitates synaptic-vesicle exocytosis by forming a tight complex with syntaxin and VAMP (also called synaptobrevin). This interaction promotes the merging of vesicle and plasma membranes enabling efficient neurotransmitter release into the synaptic cleft. SNAP25 undergoes dynamic regulation and modification which is important for precise synaptic function.

Pathways

One observes SNAP25's integration in the process of neurotransmitter release specifically in the exocytosis pathway. It associates closely with other SNARE proteins such as syntaxin-1 and VAMP-2 embodying a core element in synaptic signaling mechanisms. The proper functioning of SNAP25 within this pathway assures the controlled release of neurotransmitters influencing synaptic plasticity and communication.

SNAP25 exhibits strong associations with conditions such as Attention Deficit Hyperactivity Disorder (ADHD) and epilepsy. Variants and dysregulation in SNAP25 have been linked to problems in synaptic transmission which can contribute to these neurological disorders. The protein also interacts with other SNARE complex proteins such as syntaxin which can potentially mediate its role in disease pathogenesis. Understanding these interactions offers insights into therapeutic targets for neurological disorders involving SNAP25.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
See full target information SNAP25
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Abcam product promise

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