AB5666

Anti-SNAP25 抗体

Name: Anti-SNAP25 抗体 (ab5666) | Abcam
Brand: Abcam Limited
SKU: AB5666
Rating: 5 (5 reviews)

Anti-SNAP25 antibody

(5 Reviews)

(57 Publications)

Rabbit Polyclonal SNAP25 antibody. Suitable for ICC, Flow Cyt, WB and reacts with Rat, Mouse samples. Cited in 57 publications.

別名を表示する

Snap, Synaptosomal-associated protein 25, SNAP-25, Super protein, Synaptosomal-associated 25 kDa protein, SUP

4 Images

Supplier Data

Western blot - Anti-SNAP25 antibody (AB5666)

All lanes:

Western blot - Anti-SNAP25 antibody (ab5666)

Lane 1:

Mouse cerebellum at 30 µg

Lane 2:

Mouse brain at 30 µg

Lane 3:

Rat brain at 30 µg

Lane 4:

Membrane enriched extract at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate

Predicted band size: 23 kDa

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Supplier Data

Immunocytochemistry - Anti-SNAP25 antibody (AB5666)

Immunlocalization of SNAP-25 in cultured rat hippocampal cells using ab5666.

Unknown

Immunocytochemistry - Anti-SNAP25 antibody (AB5666)

ICC/IF image of ab5666 stained PC12 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5666, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

AbReview11837****

Western blot - Anti-SNAP25 antibody (AB5666)

All lanes:

Western blot - Anti-SNAP25 antibody (ab5666) at 1/10000 dilution

All lanes:

Whole tissue lysate prepared from mouse hippocampus

Secondary

All lanes:

HRP conjugated goat anti-rabbit Ig at 1/10000 dilution

Predicted band size: 23 kDa

Observed band size: 25 kDa

true

Exposure time: 20s

This image is courtesy of an anonymous Abreview

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat

アプリケーション

WB, Flow Cyt, ICC

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": " for 10^6 Cells", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100", "ICC-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": " for 10^6 Cells", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Goldfish": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

Preservative: 0.05% Sodium azide Constituents: 99% PBS, 0.1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SNAP25 also known as Synaptosomal-associated protein 25 plays a critical role in neurotransmitter release through its involvement in the vesicle fusion process. It is part of the SNARE complex and is essential for the docking and fusion of synaptic vesicles with the presynaptic membrane. The protein is approximately 25 kDa in mass and is expressed abundantly in neurons within the central nervous system. Among its various synonyms you may encounter 'protein SNAP' in some literature.

Biological function summary

SNARE proteins mediate the fusion of transport vesicles with their target membranes. SNAP25 as part of this complex facilitates synaptic-vesicle exocytosis by forming a tight complex with syntaxin and VAMP (also called synaptobrevin). This interaction promotes the merging of vesicle and plasma membranes enabling efficient neurotransmitter release into the synaptic cleft. SNAP25 undergoes dynamic regulation and modification which is important for precise synaptic function.

Pathways

One observes SNAP25's integration in the process of neurotransmitter release specifically in the exocytosis pathway. It associates closely with other SNARE proteins such as syntaxin-1 and VAMP-2 embodying a core element in synaptic signaling mechanisms. The proper functioning of SNAP25 within this pathway assures the controlled release of neurotransmitters influencing synaptic plasticity and communication.

SNAP25 exhibits strong associations with conditions such as Attention Deficit Hyperactivity Disorder (ADHD) and epilepsy. Variants and dysregulation in SNAP25 have been linked to problems in synaptic transmission which can contribute to these neurological disorders. The protein also interacts with other SNARE complex proteins such as syntaxin which can potentially mediate its role in disease pathogenesis. Understanding these interactions offers insights into therapeutic targets for neurological disorders involving SNAP25.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

t-SNARE involved in the molecular regulation of neurotransmitter release (PubMed : 8103915, PubMed : 8243676). May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF (PubMed : 16672379). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells (By similarity).

See full target information Snap25

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文献 (57)

Recent publications for all applications. Explore the full list and refine your search

Biology 12: PubMed37372046

2023

Brain-Derived Estrogen Regulates Neurogenesis, Learning and Memory with Aging in Female Rats.

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Unspecified application

Species

Unspecified reactive species

Yuanyuan Huang,Wuxiang Sun,Fujia Gao,Haoran Ma,Tao Yuan,Zixuan Liu,Huiyu Liu,Jiewei Hu,Jing Bai,Xin Zhang,Ruimin Wang

The Journal of pharmacology and experimental therapeutics 383:117-128 PubMed36116796

2022

Metabotropic Glutamate Receptors Modulate Exocytotic Tau Release and Propagation.

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Unspecified application

Species

Unspecified reactive species

Francesca Mazzo,Ioana Butnaru,Olivera Grubisha,Elena Ficulle,Helen Sanger,Griffin Fitzgerald,Feng Pan,Francesca Pasqui,Tracey Murray,James Monn,Xia Li,Michael Hutton,Suchira Bose,Giampietro Schiavo,Emanuele Sher

ASN neuro 14:17590914221121257 PubMed36017573

2022

Exposure to Zika Virus Results in sex-Specific Memory Deficits and Neurological Alterations in Adult Mice.

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Unspecified reactive species

Thiago A Andrade,Julia S Fahel,Jessica M de Souza,Ana C Terra,Danielle G Souza,Vivian V Costa,Mauro M Teixeira,Enrrico Bloise,Fabiola M Ribeiro

Frontiers in neuroscience 16:905403 PubMed35860293

2022

Ceftriaxone Suppresses Group II Metabotropic Glutamate Receptor Expression Contributing to Reversal of Recognition Memory Deficits of Amyloid Precursor Protein/Presenilin 1 AD Mice.

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Unspecified application

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Unspecified reactive species

ShuJuan Fan,Li Li,LiRong Liu,He Li,XiaoHui Xian,WenBin Li

Brain pathology (Zurich, Switzerland) 32:e13056 PubMed35178783

2022

Glial activation in prion diseases is selectively triggered by neuronal PrP.

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Asvin K K Lakkaraju,Silvia Sorce,Assunta Senatore,Mario Nuvolone,Jingjing Guo,Petra Schwarz,Rita Moos,Pawel Pelczar,Adriano Aguzzi

Frontiers in pharmacology 13:807330 PubMed35185566

2022

Caffeine Functions by Inhibiting Dorsal and Ventral Hippocampal Adenosine 2A Receptors to Modulate Memory and Anxiety, Respectively.

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Unspecified reactive species

Yawei Xu,Yalei Ning,Yan Zhao,Yan Peng,Fen Luo,Yuanguo Zhou,Ping Li

Central-European journal of immunology 46:305-313 PubMed34764802

2021

Tim-1 alleviates lupus nephritis-induced podocyte injury via regulating autophagy.

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Unspecified reactive species

Yunxia Yu,Caixia Zhu,Nan Yu,Lijuan Yang

Frontiers in endocrinology 12:727915 PubMed34526970

2021

Upregulation of in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control.

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Dongxiao Zhang,Satoshi Yamaguchi,Xinhao Zhang,Boxuan Yang,Naoko Kurooka,Ryosuke Sugawara,Haya Hamed H Albuayjan,Atsuko Nakatsuka,Jun Eguchi,Takeshi Y Hiyama,Atsunori Kamiya,Jun Wada

Frontiers in oncology 11:698835 PubMed34490096

2021

SNAP25 Inhibits Glioma Progression by Regulating Synapse Plasticity GLS-Mediated Glutaminolysis.

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Unspecified application

Species

Unspecified reactive species

Qiongzhen Huang,Changlin Lian,Yaoyuan Dong,Huijun Zeng,Boyang Liu,Ningbo Xu,Zhenyan He,Hongbo Guo

Cell reports 36:109333 PubMed34233191

2021

Altered conformation of α-synuclein drives dysfunction of synaptic vesicles in a synaptosomal model of Parkinson's disease.

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Unspecified application

Species

Unspecified reactive species

Luis Fonseca-Ornelas,Thibault Viennet,Matteo Rovere,Haiyang Jiang,Lei Liu,Silke Nuber,Maria Ericsson,Haribabu Arthanari,Dennis J Selkoe

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