Anti-SNAP25 抗体 (ab31281)
Key features and details
- Goat polyclonal to SNAP25
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-SNAP25 antibody
SNAP25 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to SNAP25 -
由来種
Goat -
特異性
This antibody is expected to recognise both reported isoforms NP_003072.2 (SNAP25A) and NP_570824.1 (SNAP25B). Reported variants represent identical protein: NP_001309831.1, NP_003072.2 Reported variants represent identical protein: NP_001309834.1, NP_001309832.1, NP_001309833.1, NP_001309839.1, NP_001309836.1, NP_570824.1, NP_001309838.1, NP_001309835.1, NP_001309837.1 -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Dog
-
免疫原
Synthetic peptide corresponding to Human SNAP25 aa 193-206 (C terminal).
Sequence:C-DEANQRATKMLGSG
-
ポジティブ･コントロール
- WB: Human, mouse and rat brain tissue lysate.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31281の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 1 - 2 µg/ml.
|
|
| WB | (1) |
Use a concentration of 0.005 - 0.01 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
IHC-P
Use a concentration of 1 - 2 µg/ml. |
|
WB
Use a concentration of 0.005 - 0.01 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
-
機能
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. -
組織特異性
Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. -
配列類似性
Belongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains. -
翻訳後修飾
Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association. -
細胞内局在
Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. - Information by UniProt
-
参照データベース
- Entrez Gene: 6616 Human
- Entrez Gene: 20614 Mouse
- Entrez Gene: 25012 Rat
- Omim: 600322 Human
- SwissProt: P60880 Human
- SwissProt: P60879 Mouse
- SwissProt: P60881 Rat
- Unigene: 167317 Human
see all -
別名
- bA416N4.2 antibody
- Bdr antibody
- CMS18 antibody
see all
画像
-
Western blot - Anti-SNAP25 antibody (ab31281)All lanes : Anti-SNAP25 antibody (ab31281) at 0.005 µg/ml
Lane 1 : Human Brain (Cerebellum) tissue lysate
Lanes 2-3 : Mouse Brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 23 kDaLysates in RIPA buffer. Detected by chemiluminescence.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody (ab31281)ab31281 (1µg/ml) staining of SNAP25 in paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer Ph 6, HRP-staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody (ab31281)ab31281 negative control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab31281 は 4 報の論文で使用されています。
- Mukhamedyarov MA et al. Early Alterations in Structural and Functional Properties in the Neuromuscular Junctions of Mutant FUS Mice. Int J Mol Sci 24:N/A (2023). PubMed: 37240370
- Liang D et al. Sinoatrial node pacemaker cells share dominant biological properties with glutamatergic neurons. Protein Cell 12:545-556 (2021). PubMed: 33548033
- Mizuta H et al. Additive Effects of L-Ornithine on Preferences to Basic Taste Solutions in Mice. Nutrients 13:N/A (2021). PubMed: 34836006
- Enriquez-Algeciras M et al. Deimination restores inner retinal visual function in murine demyelinating disease. J Clin Invest N/A:N/A (2013). ELISA ; Rat . PubMed: 23281397