Anti-SNAP23 抗体 (ab4114)
Key features and details
- Rabbit polyclonal to SNAP23
- Suitable for: IHC-Fr, IP, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SNAP23 antibody
SNAP23 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SNAP23 -
由来種
Rabbit -
特異性
This antibody reacts specifically with human SNAP23. -
アプリケーション
適用あり: IHC-Fr, IP, WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Fusion protein corresponding to Human SNAP23. Immunogen is a SNAP23-GST fusion protein
Database link: O00161 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4114の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration. PubMed: 17272274
|
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| IP |
Use at an assay dependent concentration. PubMed: 22393054
|
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| WB | (1) |
Use at an assay dependent concentration.
|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. PubMed: 17272274 |
|
IP
Use at an assay dependent concentration. PubMed: 22393054 |
|
WB
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion. -
組織特異性
Ubiquitous. Highest levels where found in placenta. -
配列類似性
Belongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains. -
細胞内局在
Cell membrane. Cell membrane. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8773 Human
- Entrez Gene: 20619 Mouse
- Entrez Gene: 64630 Rat
- Omim: 602534 Human
- SwissProt: O00161 Human
- SwissProt: O09044 Mouse
- SwissProt: O70377 Rat
- Unigene: 511149 Human
see all -
別名
- HsT17016 antibody
- LS-B8340 antibody
- SNAP 23 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (ab4114)
IHC image of ab4114 staining in human placenta tissue formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4114, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (22)
ab4114 は 22 報の論文で使用されています。
- Brasher MI et al. Syntaxin4-Munc18c Interaction Promotes Breast Tumor Invasion and Metastasis by Regulating MT1-MMP Trafficking. Mol Cancer Res 20:434-445 (2022). PubMed: 34876482
- Hu CY et al. Long non-coding RNA NORAD promotes the prostate cancer cell extracellular vesicle release via microRNA-541-3p-regulated PKM2 to induce bone metastasis of prostate cancer. J Exp Clin Cancer Res 40:98 (2021). PubMed: 33722248
- Qian L et al. Reduced O-GlcNAcylation of SNAP-23 promotes cisplatin resistance by inducing exosome secretion in ovarian cancer. Cell Death Discov 7:112 (2021). PubMed: 34001861
- Sikorra S et al. Engineering an Effective Human SNAP-23 Cleaving Botulinum Neurotoxin A Variant. Toxins (Basel) 12:N/A (2020). PubMed: 33352834
- Xiong X et al. Acid Sphingomyelinase regulates the localization and trafficking of palmitoylated proteins. Biol Open 8:N/A (2019). PubMed: 31142470
