Anti-SNAIL 抗体 [Sn9H2] (ab31787)
![Western blot - Anti-SNAIL antibody [Sn9H2] (ab31787)](https://www.abcam.co.jp/ps/products/31/ab31787/Images/ab31787-42947-anti-snail-antibody-sn9h2-western-blot.jpg "Western blot - Anti-SNAIL antibody [Sn9H2] (ab31787)")
Key features and details
- Rat monoclonal [Sn9H2] to SNAIL
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
製品の概要
-
製品名
Anti-SNAIL antibody [Sn9H2]
SNAIL 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [Sn9H2] to SNAIL -
由来種
Rat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Fusion protein corresponding to Human SNAIL.
-
ポジティブ･コントロール
- This antibody gave a positive signal when tested against the SNAIL recombinant protein.
-
特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
Sn9H2 -
ミエローマ
P3x63-Ag8.653 -
アイソタイプ
IgG2a -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31787の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 10 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
|
| 特記事項 |
|---|
|
WB
Use a concentration of 10 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
ターゲット情報
-
機能
Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself. -
組織特異性
Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. -
配列類似性
Belongs to the snail C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers. -
翻訳後修飾
Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.
Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2.
O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.
ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation. -
細胞内局在
Nucleus. Cytoplasm. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs. - Information by UniProt
-
参照データベース
- Entrez Gene: 6615 Human
- Omim: 604238 Human
- SwissProt: O95863 Human
- Unigene: 48029 Human
-
別名
- dJ710H13.1 antibody
- Protein sna antibody
- Protein snail homolog 1 antibody
see all
画像
-
Western blot - Anti-SNAIL antibody [Sn9H2] (ab31787)All lanes : Anti-SNAIL antibody [Sn9H2] (ab31787) at 10 µg/ml
Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
Lane 2 : SLUG (SNAI2) Human Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutes
Ab31787 recognizes the tagged recombinant SNAIL protein which has an expected molecular weight of 68 kDa.
データシートおよび資料
-
Datasheet download
参考文献 (8)
ab31787 は 8 報の論文で使用されています。
- Zhang X et al. Over-Expression of ARID3B Suppresses Tumor Progression and Predicts Better Prognosis in Patients With Gastric Cancer. Cancer Control 30:10732748231169403 (2023). PubMed: 37071790
- Yi JM et al. Human Neuralized is a novel tumour suppressor targeting Wnt/β-catenin signalling in colon cancer. EMBO Rep 24:e56335 (2023). PubMed: 37341560
- Zhang Y et al. LINC00891 regulated by miR-128-3p/GATA2 axis impedes lung cancer cell proliferation, invasion and EMT by inhibiting RhoA pathway. Acta Biochim Biophys Sin (Shanghai) 54:378-387 (2022). PubMed: 35538035
- Tang Z et al. Enhanced monoacylglycerol lipolysis by ABHD6 promotes NSCLC pathogenesis. EBioMedicine 53:102696 (2020). PubMed: 32143183
- Wang S et al. MiR-193a-5p suppresses cell proliferation and induces cell apoptosis by regulating HOXA7 in human ovarian cancer. Neoplasma 67:825-833 (2020). PubMed: 32305054
- Weng W et al. Metastasis-associated protein 1 promotes tumor invasion by downregulation of E-cadherin. Int J Oncol 44:812-8 (2014). Human . PubMed: 24424621
- Fedorova L et al. Down-regulation of the transcription factor snail in the placentas of patients with preeclampsia and in a rat model of preeclampsia. Reprod Biol Endocrinol 10:15 (2012). PubMed: 22360878
- Rosivatz E et al. Expression and nuclear localization of Snail, an E-cadherin repressor, in adenocarcinomas of the upper gastrointestinal tract. Virchows Arch 448:277-87 (2006). PubMed: 16328348
