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AB31787

Anti-SNAIL 抗体 [Sn9H2]

Anti-SNAIL antibody [Sn9H2]

1

(1 Review)

|

(8 Publications)

Rat Monoclonal SNAIL antibody. Suitable for WB and reacts with Recombinant full length protein - Human samples. Cited in 8 publications.

別名を表示する

SNAH, SNAI1, Zinc finger protein SNAI1, Protein snail homolog 1, Protein sna

1 Images
Western blot - Anti-SNAIL antibody [Sn9H2] (AB31787)
  • WB

Project

Western blot - Anti-SNAIL antibody [Sn9H2] (AB31787)

ab31787 recognizes the tagged recombinant SNAIL protein which has an expected molecular weight of 68 kDa.

All lanes:

Western blot - Anti-SNAIL antibody [Sn9H2] (ab31787) at 10 µg/mL

Lane 1:

SNAIL (SNAI1) Human Recombinant Protein at 0.1 µg

Lane 2:

SLUG (SNAI2) Human Recombinant Protein at 0.1 µg

Secondary

All lanes:

Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution

Predicted band size: 29 kDa

Observed band size: 68 kDa

false

Exposure time: 16min

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

Sn9H2

アイソタイプ

IgG2a

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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下記製品にもご興味をお持ちいただけるかもしれません:

AB314363

Human SNAIL ELISA Kit

0

0 Reviews

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製品の詳細

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SNAIL also known as SNAI1 is a zinc finger transcription factor involved in the regulation of cellular processes. The SNAIL protein has a mass of approximately 29 kDa and is robustly expressed in various tissues including embryonic tissues and cancerous cells. The protein functions as a repressor of transcription influencing the expression of genes associated with cellular adhesion and movement. Due to its integral role SNAIL is involved in complex regulatory networks that control cell fate and differentiation.
Biological function summary

SNAIL contributes to the epithelial-mesenchymal transition (EMT) a process critical for embryogenesis and tumor progression. It forms a part of a complex network of transcription factors that regulate cell-cell adhesion molecules like E-cadherin. SNAIL's ability to bind to specific DNA sequences allows it to suppress or activate the transcription of target genes promoting cellular metamorphosis and enabling cells to acquire motility and invade other tissues.

Pathways

SNAIL operates significantly within the TGF-beta and Wnt signaling pathways. The TGF-beta pathway enhances the expression of SNAIL which in turn represses genes that maintain the epithelial phenotype. The Wnt pathway also modulates SNAIL activity connecting it with proteins such as beta-catenin to drive EMT. These pathways highlight SNAIL's involvement in sophisticated signaling pathways that determine cell behavior adaptation and tissue remodeling.

Overexpression of SNAIL associates with cancer progression particularly in metastasis due to its role in EMT. It connects with proteins like Slug and ZEB1 in this context enhancing the invasive capabilities of cancer cells. Moreover SNAIL is implicated in fibrotic diseases where excessive tissue scarring occurs. In such conditions TGF-beta-mediated activation of SNAIL contributes to abnormal tissue remodeling and fibrosis. The involvement of SNAIL in these diseases marks it as a potential target for therapeutic intervention.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration (PubMed : 10655587, PubMed : 15647282, PubMed : 20389281, PubMed : 20562920, PubMed : 21952048, PubMed : 25827072). Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription (PubMed : 10655587, PubMed : 20389281, PubMed : 20562920). The N-terminal SNAG domain competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (in vitro) (PubMed : 20389281, PubMed : 21300290, PubMed : 23721412). During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (PubMed : 16096638). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3 (PubMed : 20121949). In addition, may also activate the CDKN2B promoter by itself (PubMed : 20121949).
See full target information SNAI1
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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 24:e56335 PubMed37341560

2023

Human Neuralized is a novel tumour suppressor targeting Wnt/β-catenin signalling in colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Joo Mi Yi,Tae-Hong Kang,Yu Kyeong Han,Ha Young Park,Ju Hwan Yang,Jin-Han Bae,Jung-Soo Suh,Tae-Jin Kim,Joong-Gook Kim,Yan-Hong Cui,Hiromu Suzuki,Kohei Kumegawa,Sung Joo Kim,Yi Zhao,In Ja Park,Seung-Mo Hong,Joon-Yong Chung,Su-Jae Lee

Cancer control : journal of the Moffitt Cancer Center 30:10732748231169403 PubMed37071790

2023

Over-Expression of ARID3B Suppresses Tumor Progression and Predicts Better Prognosis in Patients With Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xunlei Zhang,Xinyue Qiu,Wenjing Zhao,Li Song,Xingsong Zhang,Lei Yang,Min Tao

Acta biochimica et biophysica Sinica 54:378-387 PubMed35538035

2022

LINC00891 regulated by miR-128-3p/GATA2 axis impedes lung cancer cell proliferation, invasion and EMT by inhibiting RhoA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunfeng Zhang,Dingli Song,Ziyang Peng,Rui Wang,Kai Li,Hong Ren,Xin Sun,Ning Du,Shou-Ching Tang

Neoplasma 67:825-833 PubMed32305054

2020

MiR-193a-5p suppresses cell proliferation and induces cell apoptosis by regulating HOXA7 in human ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

S Wang,Y J Diao,B B Zhu

EBioMedicine 53:102696 PubMed32143183

2020

Enhanced monoacylglycerol lipolysis by ABHD6 promotes NSCLC pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyuan Tang,Hao Xie,Christoph Heier,Jianfei Huang,Qiuling Zheng,Thomas O Eichmann,Gabriele Schoiswohl,Jun Ni,Rudolf Zechner,Songshi Ni,Haiping Hao

International journal of oncology 44:812-8 PubMed24424621

2014

Metastasis-associated protein 1 promotes tumor invasion by downregulation of E-cadherin.

Applications

Unspecified application

Species

Human

Wenhao Weng,Jiayi Yin,Yue Zhang,Jin Qiu,Xinghe Wang

Reproductive biology and endocrinology : RB&E 10:15 PubMed22360878

2012

Down-regulation of the transcription factor snail in the placentas of patients with preeclampsia and in a rat model of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Larisa Fedorova,Cara Gatto-Weis,Sleiman Smaili,Nauman Khurshid,Joseph I Shapiro,Deepak Malhotra,Terrence Horrigan

Virchows Archiv : an international journal of pathology 448:277-87 PubMed16328348

2005

Expression and nuclear localization of Snail, an E-cadherin repressor, in adenocarcinomas of the upper gastrointestinal tract.

Applications

Unspecified application

Species

Unspecified reactive species

Erika Rosivatz,Karl-Friedrich Becker,Elisabeth Kremmer,Christina Schott,Kareen Blechschmidt,Heinz Höfler,Mario Sarbia
View all publications
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Abcam product promise

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