Anti-SNAIL + SLUG (phospho S246) 抗体 (ab63568)
Key features and details
- Rabbit polyclonal to SNAIL + SLUG (phospho S246)
- Suitable for: WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
製品の概要
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製品名
Anti-SNAIL + SLUG (phospho S246) antibody
SNAIL + SLUG 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SNAIL + SLUG (phospho S246) -
由来種
Rabbit -
特異性
ab63568 detects endogenous levels of SNAIL only when phosphorylated at serine 246. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human, Recombinant fragment
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide corresponding to Human SNAIL + SLUG (phospho S246).
Database link: O95863 -
ポジティブ･コントロール
- H29 nuclear lysate HuvEc cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63568の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa).
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| ICC/IF |
1/500 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa). |
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ICC/IF
1/500 - 1/1000. |
ターゲット情報
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関連性
Function: SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. PTM: SNAIL is phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Similarity: Both SNAIL and SLUG belong to the snail C2H2-type zinc-finger protein family. Tissue specificity: SNAIL is expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. SLUG is expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. -
細胞内局在
Slug is generally nuclear, while Snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs. -
参照データベース
- Entrez Gene: 6591 Human
- Entrez Gene: 6615 Human
- Entrez Gene: 20583 Mouse
- Entrez Gene: 20613 Mouse
- Omim: 604238 Human
- SwissProt: O43623 Human
- SwissProt: O95863 Human
- SwissProt: P97469 Mouse
see all -
別名
- dJ710H13.1 antibody
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
see all
画像
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Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)All lanes : Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) at 1/500 dilution
Lane 1 : Extracts from HT29 cells (5-30µg), minus immunising peptide
Lane 2 : Extracts from HT29 cells (5-30µg) plus immunising peptide (5-10µg)
Predicted band size: 29 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted? -
Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)All lanes : Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) at 1/500 dilution
Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
Lane 2 : SLUG (SNAI2) Human Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Ab63568 recognizes the tagged recombinant SLUG and SNAIL proteins which have an expected molecular weight of 68 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)Immunofluorescent staining of HuvEc cells, using ab63568 at a 1/500 dilution, minus immunising peptide (left image) and plus immunising peptide (right image).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab63568 は 7 報の論文で使用されています。
- Pascual AS et al. Dual inhibition of TGFßR and ROCK reverses the epithelial to mesenchymal transition in collectively migrating zebrafish keratocytes. Cell Biol Int 45:1288-1295 (2021). PubMed: 33710707
- Xu Z et al. S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn. Aging (Albany NY) 13:15523-15537 (2021). PubMed: 34099591
- Liu L et al. A Slug-dependent mechanism is responsible for tumor suppression of p53-stabilizing compound CP-31398 in p53-mutated endometrial carcinoma. J Cell Physiol 235:8768-8778 (2020). PubMed: 32633026
- Chow HY et al. Group I Paks are essential for epithelial- mesenchymal transition in an Apc-driven model of colorectal cancer. Nat Commun 9:3473 (2018). PubMed: 30150766
- Yan H et al. Long noncoding RNA MLK7-AS1 promotes ovarian cancer cells progression by modulating miR-375/YAP1 axis. J Exp Clin Cancer Res 37:237 (2018). PubMed: 30249278
- Tong R et al. KCTD11 inhibits growth and metastasis of hepatocellular carcinoma through activating Hippo signaling. Oncotarget 8:37717-37729 (2017). PubMed: 28465479
- Magnusson C et al. Regulation of cysteinyl leukotriene receptor 2 expression-a potential anti-tumor mechanism. PLoS One 6:e29060 (2011). WB ; Human . PubMed: 22194989