Anti-SNAIL + SLUG 抗体 [20C8] (ab78105)
![Western blot - Anti-SNAIL + SLUG antibody [20C8] (ab78105)](https://www.abcam.co.jp/ps/products/78/ab78105/Images/ab78105-93900-anti-snail-slug-antibody-20c8-western-blot.jpg "Western blot - Anti-SNAIL + SLUG antibody [20C8] (ab78105)")
Key features and details
- Mouse monoclonal [20C8] to SNAIL + SLUG
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-SNAIL + SLUG antibody [20C8]
SNAIL + SLUG 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [20C8] to SNAIL + SLUG -
由来種
Mouse -
特異性
This antibody detects endogonous proteins in the following cell lines: MDAMB-453; MDAMB-435; MDAMB-231; A375.
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アプリケーション
適用あり: Sandwich ELISA, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein (Human)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
20C8 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab78105の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 1 µg/ml. For sandwich ELISA, use this antibody as Capture at 1 µg/ml with Rabbit polyclonal to SNAIL - ChIP Grade (ab85931) as Detection.
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| WB | (1) |
1/250. Predicted molecular weight: 29 kDa.
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| 特記事項 |
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Sandwich ELISA
Use a concentration of 1 µg/ml. For sandwich ELISA, use this antibody as Capture at 1 µg/ml with Rabbit polyclonal to SNAIL - ChIP Grade (ab85931) as Detection. |
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WB
1/250. Predicted molecular weight: 29 kDa. |
ターゲット情報
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関連性
Function: SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. PTM: SNAIL is phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Similarity: Both SNAIL and SLUG belong to the snail C2H2-type zinc-finger protein family. Tissue specificity: SNAIL is expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. SLUG is expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. -
細胞内局在
Slug is generally nuclear, while Snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs. -
参照データベース
- Entrez Gene: 6591 Human
- Entrez Gene: 6615 Human
- Omim: 604238 Human
- SwissProt: O43623 Human
- SwissProt: O95863 Human
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別名
- dJ710H13.1 antibody
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
see all
画像
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Western blot - Anti-SNAIL + SLUG antibody [20C8] (ab78105)All lanes : Anti-SNAIL + SLUG antibody [20C8] (ab78105) at 1/250 dilution
Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
Lane 2 : SLUG (SNAI2) Human Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Ab78105 recognizes the tagged recombinant SLUG and SNAIL proteins which have an expected molecular weight of 68 kDa -
![Sandwich ELISA - Anti-SNAIL + SLUG antibody [20C8] (ab78105)](https://www.abcam.co.jp/ps/products/78/ab78105/Images/ab78105-93899-anti-snail-slug-antibody-20c8-sandwich-elisa.jpg)
Sandwich ELISA - Anti-SNAIL + SLUG antibody [20C8] (ab78105)Standard Curve for SNAIL ; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [20C8] to SNAIL + SLUG (ab78105) at 1µg/ml and Detector Antibody Rabbit polyclonal to SNAIL + SLUG - ChIP Grade (ab85931) at 0.5µg/ml.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab78105 は 5 報の論文で使用されています。
- Gao N et al. SNAI1 interacts with HDAC1 to control TGF-ß2-induced epithelial-mesenchymal transition in human lens epithelial cells. Int J Mol Med 45:265-273 (2020). PubMed: 31746377
- Li J et al. Activation of autophagy inhibits epithelial to mesenchymal transition process of human lens epithelial cells induced by high glucose conditions. Cell Signal 75:109768 (2020). PubMed: 32896607
- Yang HD et al. T-cell immune regulator 1 enhances metastasis in hepatocellular carcinoma. Exp Mol Med 50:e420 (2018). PubMed: 29303507
- Goldman O et al. The mesenchymal transcription factor SNAI-1 instructs human liver specification. Stem Cell Res 17:62-8 (2016). ChIP ; Human . PubMed: 27240252
- Ju SY et al. Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells. Cell Death Dis 6:e1801 (2015). WB . PubMed: 26136074