Anti-SMYD1 抗体 [EPR13574(B)-30] (ab181372)
![Western blot - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)](https://www.abcam.co.jp/ps/products/181/ab181372/Images/ab181372-210752-anti-smyd1-antibody-epr13574b-30-western-blot.jpg "Western blot - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13574(B)-30] to SMYD1
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SMYD1 antibody [EPR13574(B)-30]
SMYD1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13574(B)-30] to SMYD1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IPmore details
適用なし: ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human skeletal muscle and fetal heart lysates; Human heart tissue and skeletal muscle tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13574(B)-30 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181372の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 57 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
1/10 - 1/100.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 57 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IP
1/10 - 1/100. |
ターゲット情報
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機能
Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis. -
配列類似性
Contains 1 MYND-type zinc finger.
Contains 1 SET domain. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 150572 Human
- Entrez Gene: 12180 Mouse
- Entrez Gene: 297333 Rat
- Omim: 606846 Human
- SwissProt: Q8NB12 Human
- SwissProt: P97443 Mouse
- Unigene: 516176 Human
- Unigene: 234274 Mouse
see all -
別名
- BOP antibody
- CD8 beta opposite antibody
- CD8b opposite antibody
see all
画像
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Western blot - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)All lanes : Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human skeletal muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 57 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)](https://www.abcam.co.jp/ps/products/181/ab181372/Images/ab181372-210751-anti-smyd1-antibody-epr13574b-30-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling SMYD1 with ab181372 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)](https://www.abcam.co.jp/ps/products/181/ab181372/Images/ab181372-210750-anti-smyd1-antibody-epr13574b-30-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling SMYD1 with ab181372 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)](https://www.abcam.co.jp/ps/products/181/ab181372/Images/ab181372-210749-anti-smyd1-antibody-epr13574b-30-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal heart lysate or (Lane 2) 1XPBS (negative control) immunoprecipitated using ab181372 at 1/10 dilution with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)](https://www.abcam.co.jp/ps/products/181/ab181372/Images/ab181372-5-benefits-of-recombinant-antibodies.png)
Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab181372 は 2 報の論文で使用されています。
- Sakamoto T et al. The nuclear receptor ERR cooperates with the cardiogenic factor GATA4 to orchestrate cardiomyocyte maturation. Nat Commun 13:1991 (2022). PubMed: 35418170
- Sauls K et al. Initiating Events in Direct Cardiomyocyte Reprogramming. Cell Rep 22:1913-1922 (2018). WB ; Mouse . PubMed: 29444441