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リコンビナントRabMAb

Anti-SMURF1 抗体 [EPR25111-116] (ab300408)

Submit a review Submit a question References (1)
Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408)
  • Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408)
  • Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408)
  • Anti-SMURF1 antibody [EPR25111-116] (AB300408)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25111-116] to SMURF1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-SMURF1 antibody [EPR25111-116]
    SMURF1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25111-116] to SMURF1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt (Intra),ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Whole cell lysates: MCF7 (human breast adenocarcinoma epithelial cell) transfected with scrambled siRNA control, MCF7 transfected with siRNA specifically targeting Smurf1, HeLa (human epithelial cell line from cervix adenocarcinoma), 293T (human embryonic kidney epithelial cell), NIH/3T3 (mouse embryonic fibroblast), PC-12 (rat adrenal gland pheochromocytoma). HeLa (human epithelial cell line from cervical adenocarcinoma), whole cell fresh lysate.

  • 特記事項

     ab300408 does not react in: IHC-P with human, mouse, and rat; ICC, intracellular flow cytometry and immunoprecipitation with human. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR25111-116

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300408の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 86 kDa).
特記事項
WB
1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 86 kDa).
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Acts by mediating ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members.

  • パスウェイ

    Protein modification; protein ubiquitination.

  • 配列類似性

    Contains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 2 WW domains.

  • 翻訳後修飾

    Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • E3 ubiquitin-protein ligase SMURF1 antibody
      • hSMURF1 antibody
      • KIAA1625 antibody
      • Smad specific E3 ubiquitin ligase 1 antibody
      • SMAD specific E3 ubiquitin protein ligase 1 antibody
      • Smad ubiquitination regulatory factor 1 antibody
      • SMAD-specific E3 ubiquitin-protein ligase 1 antibody
      • SMUF1_HUMAN antibody
      • SMURF 1 antibody
      • smurf1 antibody
      see all

    画像

    • Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408)
      Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408)
      All lanes : Anti-SMURF1 antibody [EPR25111-116] (ab300408) at 1/1000 dilution

      Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate
      Lane 2 : MCF7 transfected with siRNA specifically targeting Smurf1, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 86 kDa
      Observed band size: 87 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and dilution buffer and concentration: 5% NFDM/TBST

      This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range. 

    • Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408)
      Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408)
      All lanes : Anti-SMURF1 antibody [EPR25111-116] (ab300408) at 1/1000 dilution

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
      Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
      Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
      Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 86 kDa
      Observed band size: 87 kDa why is the actual band size different from the predicted?


      Exposure time: 136 seconds


      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    • Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408)
      Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408)
      All lanes : Anti-SMURF1 antibody [EPR25111-116] (ab300408) at 1/1000 dilution

      Lane 1 : HeLa (human epithelial cell line from cervical adenocarcinoma), whole cell fresh lysate
      Lane 2 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 86 kDa
      Observed band size: 87 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      In lane 1, lysate was freshlymade and used for Western Blotting immediately to minimize protein degradation. In lane 2, the lysate was stored at -80? prior to Western Blotting.

      The blot of lane 2 was developed using a high sensitivity ECL substrate.

      Exposure time: Lane 1: 158 seconds, Lane 2: 3 minutes

    • Anti-SMURF1 antibody [EPR25111-116] (AB300408)
      Anti-SMURF1 antibody [EPR25111-116] (AB300408)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab300408 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300408 は 1 報の論文で使用されています。

    • Cao L  et al. Expression and regulatory network of E3 ubiquitin ligase NEDD4 family in cancers. BMC Cancer 23:526 (2023). PubMed: 37291499

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