AB300408

Anti-SMURF1 抗体 [EPR25111-116]

Anti-SMURF1 antibody [EPR25111-116]

20ul selling size
RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal SMURF1 antibody. Suitable for WB and reacts with Rat, Mouse, Human, Transfected cell line samples. Cited in 3 publications.

別名を表示する

KIAA1625, SMURF1, E3 ubiquitin-protein ligase SMURF1, hSMURF1, HECT-type E3 ubiquitin transferase SMURF1, SMAD ubiquitination regulatory factor 1, SMAD-specific E3 ubiquitin-protein ligase 1

4 Images

Lab

Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Smurf1 is degraded through the ubiquitin–proteasome system(PMID : 20580715).

All lanes:

Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

786-O (Human kidney renal cell adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 5:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 6:

Mouse lung tissue lysate at 20 µg

Lane 7:

Mouse liver tissue lysate at 20 µg

Lane 8:

Rat lung tissue lysate at 20 µg

Lane 9:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa,87 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

In lane 1, lysate was freshlymade and used for Western Blotting immediately to minimize protein degradation. In lane 2, the lysate was stored at -80℃ prior to Western Blotting.

The blot of lane 2 was developed using a high sensitivity ECL substrate.

Exposure time : Lane 1 : 158 seconds, Lane 2 : 3 minutes

All lanes:

Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 86 kDa

Observed band size: 87 kDa

false

Supplier Data

Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408)

Blocking and dilution buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate at 20 µg

Lane 2:

MCF7 transfected with siRNA specifically targeting Smurf1, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 86 kDa

Observed band size: 87 kDa

true

Exposure time: 3min

Lab

Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Smurf1 is degraded through the ubiquitin–proteasome system(PMID : 20580715).

All lanes:

Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408) at 1/1000 dilution

Lane 1:

Fresh mouse liver tissue lysate at 20 µg

Lane 2:

Frozen mouse liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa

false

Exposure time: 180s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR25111-116

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Mouse, Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Transfected cell line": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SMURF1 also known as SMAD-specific E3 ubiquitin protein ligase 1 functions as an E3 ubiquitin ligase involved in the targeted degradation of proteins. This protein weighs approximately 72 kDa and expresses primarily in various human tissues including bone lung and kidney. SMURF1 plays an essential role in regulating protein turnover by tagging substrates for proteasomal degradation. It interacts with substrates through a C2 domain which aids in membrane localization and substrate recognition effectively controlling cellular protein levels and their functions.

Biological function summary

SMURF1 influences numerous cellular processes such as bone morphogenetic protein (BMP) signaling by regulating the availability and stability of signaling molecules. It does not function in isolation; it is a component of a ubiquitin-proteasome system complex that includes other cofactors assisting its activity. SMURF1 therefore affects cell growth differentiation and homeostasis by managing signal transduction pathways critical for these cellular activities.

Pathways

SMURF1 is involved in the BMP and Wnt signaling pathways. Both are fundamental for various biological activities including embryonic development and cell cycle regulation. Within these pathways SMURF1 influences the activity of SMAD6 and related proteins modulating their turnover and impact on downstream signaling processes. These pathways also interact with other cellular pathways but SMURF1's specific role remains concentrated on modulating protein ubiquitination events.

SMURF1 has been linked to cancer and osteoporosis. Its dysregulation can disrupt normal cellular signaling contributing to tumorigenesis or impaired bone maintenance. In cancer its interaction with proteins such as SMAD7 can alter cell signaling promoting uncontrolled cell growth. In osteoporosis SMURF1's regulation of bone remodeling processes might be compromised leading to decreased bone mass and fragility. Understanding SMURF1's roles may open avenues for therapeutic interventions in these disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed : 23087404). Acts as an antagonist of TGF-beta signaling by ubiquitinating TGFBR1 and targeting it for degradation (PubMed : 21791611). Plays a role in dendrite formation by melanocytes (PubMed : 23999003).

See full target information SMURF1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 15:124 PubMed40877964

2025

Enriched environment inhibits GLT-1 ubiquitination by downregulating SMURF1 to attenuate ischemic brain injury induced excitotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhang,Wen Liu,Tao Huang,Lingling Liu,Xiuping Chen

Journal of experimental & clinical cancer research : CR 43:208 PubMed39061061

2024

SLC14A1 and TGF-β signaling: a feedback loop driving EMT and colorectal cancer metachronous liver metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yixun Zhang,Yumeng Yang,Xuan Qi,Peng Cui,Yi Kang,Haiyi Liu,Zhigang Wei,Haibo Wang

BMC cancer 23:526 PubMed37291499

2023

Expression and regulatory network of E3 ubiquitin ligase NEDD4 family in cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Liangzi Cao,Hao Li,Xiaofang Liu,Yubang Wang,Bowen Zheng,Chengzhong Xing,Naijin Zhang,Jingwei Liu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SMURF1 抗体 [EPR25111-116]

Anti-SMURF1 antibody [EPR25111-116]

Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408)

Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408)

Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408)

Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise