Anti-Smg1 抗体 (ab80600)
Key features and details
- Rabbit polyclonal to Smg1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Smg1 antibody
Smg1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Smg1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rabbit, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Orangutan, Platypus
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免疫原
Synthetic peptide within Human Smg1 aa 1250-1300. The exact sequence is proprietary.
Database link: NP_055907.3 -
ポジティブ･コントロール
- HeLa cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.764% Sodium citrate, 1.815% Tris, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab80600の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 410 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 410 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ. -
組織特異性
Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver. -
配列類似性
Belongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 1 HEAT repeat.
Contains 1 PI3K/PI4K domain. -
翻訳後修飾
Autophosphorylated. -
細胞内局在
Nucleus. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 23049 Human
- Entrez Gene: 233789 Mouse
- Omim: 607032 Human
- SwissProt: Q96Q15 Human
- SwissProt: Q8BKX6 Mouse
- Unigene: 460179 Human
- Unigene: 309053 Mouse
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別名
- 61E3.4 antibody
- ATX antibody
- hSMG-1 antibody
see all
画像
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Western blot - Anti-Smg1 antibody (ab80600)All lanes : Anti-Smg1 antibody (ab80600) at 1 µg/ml
Lane 1 : Whole HeLa cell lysate at 50 µg
Lane 2 : Whole HeLa cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 410 kDa
Observed band size: 410 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Chemiluminescence with exposure times of 30 seconds -
Immunoprecipitation - Anti-Smg1 antibody (ab80600)Detection of Human Smg1 by Immunoprecipitation. Sample: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. ab80600 used for IP at 3 mcg/mg lysate. Subsequent Wb detection with ab80600 used at 1 mcg/ml. Chemiluminescence with exposure times of 3 mins
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab80600 は 1 報の論文で使用されています。
- Henderson-Smith A et al. SMG1 identified as a regulator of Parkinson's disease-associated alpha-synuclein through siRNA screening. PLoS One 8:e77711 (2013). WB . PubMed: 24204929