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Anti-SMCHD1 抗体 (ab31865)

Reviews (2)Q&A (2)References (20)
Immunocytochemistry/ Immunofluorescence - Anti-SMCHD1 antibody (ab31865)
  • Immunocytochemistry/ Immunofluorescence - Anti-SMCHD1 antibody (ab31865)

Key features and details

  • Rabbit polyclonal to SMCHD1
  • Suitable for: ICC/IF, WB, ChIP
  • Reacts with: Mouse, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-SMCHD1 antibody
    SMCHD1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to SMCHD1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, ChIPmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Synthetic peptide corresponding to Human SMCHD1 aa 1750-1850 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab31864)

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 98.98% PBS, 1% BSA

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab31865の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF (1)
1/100.
WB (1)
Use at an assay dependent concentration. Predicted molecular weight: 226 kDa. PubMed: 21836164
ChIP
Use at an assay dependent concentration. PubMed: 28587678
特記事項
ICC/IF
1/100.
WB
Use at an assay dependent concentration. Predicted molecular weight: 226 kDa. PubMed: 21836164
ChIP
Use at an assay dependent concentration. PubMed: 28587678

ターゲット情報

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-SMCHD1 antibody (ab31865)
    Immunocytochemistry/ Immunofluorescence - Anti-SMCHD1 antibody (ab31865)This image is courtesy of an Abreview submitted by Dr Kirk McManus
     ab31865 (1/100) staining SMCHD1 by immunofluorescence in assynchronous HeLa cells (green). Secondary antibody: goat polyclonal antibody conjugated to Cy3; Counterstain: DAPI (red). Please refer to Abreview for further information.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-SMCHD1 antibody (ab31865)
    Immunocytochemistry/ Immunofluorescence - Anti-SMCHD1 antibody (ab31865)
    ICC/IF image of ab31865 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31865, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methaol fixed (5 min) HeLa, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.

参考文献 (20)

ab31865 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab31865 は 20 報の論文で使用されています。

  • Laberthonnière C  et al. In skeletal muscle and neural crest cells, SMCHD1 regulates biological pathways relevant for Bosma syndrome and facioscapulohumeral dystrophy phenotype. Nucleic Acids Res 51:7269-7287 (2023). PubMed: 37334829
  • Šikrová D  et al. SMCHD1 and LRIF1 converge at the FSHD-associated D4Z4 repeat and LRIF1 promoter yet display different modes of action. Commun Biol 6:677 (2023). PubMed: 37380887
  • Tapia Del Fierro A  et al. SMCHD1 has separable roles in chromatin architecture and gene silencing that could be targeted in disease. Nat Commun 14:5466 (2023). PubMed: 37749075
  • Huang Z  et al. The chromosomal protein SMCHD1 regulates DNA methylation and the 2c-like state of embryonic stem cells by antagonizing TET proteins. Sci Adv 7:N/A (2021). PubMed: 33523915
  • Theurillat I  et al. Extensive SUMO Modification of Repressive Chromatin Factors Distinguishes Pluripotent from Somatic Cells. Cell Rep 32:108146 (2020). PubMed: 32937131
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-SMCHD1 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Loading amount
50 µg
Specification
MEF
Gel Running Conditions
Reduced Denaturing (7%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 20 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-SMCHD1 antibody

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

投稿 Feb 21 2007

Question

I will also be detecting murine CAP-H (BRRN1). Is it possible to be enrolled in AbTrial program if I order ab136973 and/or ab154105?

Read More
Answer

I am very pleased to hear you would like to test ab31865, ab122555, ab136973, and ab154105 in mouse. These codes will give you the value listed above off future orders before the expiration date. To redeem this offer, please submit an Abreview for mouse for each antibody and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

Read More

Question

I am
interested in ordering ab31865 and ab122555 but am not sure if these two antibodies work well in recognizing murine
Smchd1. Is it possible to order smaller aliquots of these two antibodies so that I can do some preliminary experiments before purchasing the whole vial? Please let me know.

Read More
Answer

Thank you very much for your interest our products.

To our knowledge, ab31865 and ab122555 have not been tested in mouse. Since we carry over 100,000 products, we are unable to store multiple vial sizes for samples. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing ab31865 or ab122555. We would expect both antibodies to cross-react with mouse due to high sequence homology (> 90%) so just let us know which one you would like to test.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com