Anti-SMC2 抗体 (ab10412)
Key features and details
- Rabbit polyclonal to SMC2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-SMC2 antibody
SMC2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SMC2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
-
免疫原
Synthetic peptide. The epitope maps to a region between residue 1150 and the C-terminus (residue 1197) of human Structural Maintenance of Chromosomes 2 using the numbering given in entry NP_006435.1 (GeneID 10592).
-
ポジティブ･コントロール
- IP: HeLa cell lysates; WB: HeLa and HEK293 cell lysates
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antibodies were affinity purified using the peptide immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10412の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 137 kDa).
|
|
| IP |
Use a concentration of 1 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 137 kDa). |
|
IP
Use a concentration of 1 µg/ml. |
ターゲット情報
-
機能
Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. -
配列類似性
Belongs to the SMC family. SMC2 subfamily. -
ドメイン
The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC4, forming a V-shaped heterodimer. -
細胞内局在
Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase. - Information by UniProt
-
参照データベース
- Entrez Gene: 10592 Human
- Omim: 605576 Human
- SwissProt: O95347 Human
- Unigene: 119023 Human
-
別名
- CAP E antibody
- CAPE antibody
- Chromosome associated protein E antibody
see all
画像
-
Western blot - Anti-SMC2 antibody (ab10412)All lanes : Anti-SMC2 antibody (ab10412) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HEK293 cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 137 kDa
Exposure time: 3 seconds -
Immunoprecipitation - Anti-SMC2 antibody (ab10412)Immunoprecipitation of Human Aurora A with ab10412 at 1 µg/mL.
Lane 1: ab10412 + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.Lane 2: IgG control + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Detection: Chemiluminescence
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (22)
ab10412 は 22 報の論文で使用されています。
- Olins AL et al. Hyperosmotic stress: in situ chromatin phase separation. Nucleus 11:1-18 (2020). PubMed: 31924112
- Pinzaru AM et al. Replication stress conferred by POT1 dysfunction promotes telomere relocalization to the nuclear pore. Genes Dev 34:1619-1636 (2020). PubMed: 33122293
- Venegas AB et al. Inducible Degradation of the Human SMC5/6 Complex Reveals an Essential Role Only during Interphase. Cell Rep 31:107533 (2020). PubMed: 32320646
- Yang Y et al. NudCL2 is an Hsp90 cochaperone to regulate sister chromatid cohesion by stabilizing cohesin subunits. Cell Mol Life Sci 76:381-395 (2019). PubMed: 30368549
- Champion L et al. Dissociation of membrane-chromatin contacts is required for proper chromosome segregation in mitosis. Mol Biol Cell 30:427-440 (2019). PubMed: 30586323