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AB10412

Anti-SMC2 抗体

Anti-SMC2 antibody

4

(1 Review)

|

(22 Publications)

Rabbit Polyclonal SMC2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 22 publications. Immunogen corresponding to Synthetic Peptide within Human SMC2.

別名を表示する

CAPE, SMC2L1, PRO0324, SMC2, Structural maintenance of chromosomes protein 2, SMC protein 2, SMC-2, Chromosome-associated protein E, XCAP-E homolog, hCAP-E

2 Images
Immunoprecipitation - Anti-SMC2 antibody (AB10412)
  • IP

Supplier Data

Immunoprecipitation - Anti-SMC2 antibody (AB10412)

Immunoprecipitation of Human Aurora A with ab10412 at 1 μg/mL.

Lane 1 : ab10412 + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

Lane 2 : IgG control + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

Detection : Chemiluminescence

All lanes:

Immunoprecipitation - Anti-SMC2 antibody (ab10412)

Predicted band size: 135 kDa

false

Exposure time: 3s

Western blot - Anti-SMC2 antibody (AB10412)
  • WB

Supplier Data

Western blot - Anti-SMC2 antibody (AB10412)

All lanes:

Western blot - Anti-SMC2 antibody (ab10412) at 0.1 µg/mL

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

HEK293 cell lysate at 50 µg

Predicted band size: 135 kDa

false

Exposure time: 3s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IP, WB

applications

免疫原

Synthetic Peptide within Human SMC2. The exact immunogen used to generate this antibody is proprietary information.

O95347

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
Antibodies were affinity purified using the peptide immobilized on solid support.
バッファー組成
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SMC2 also known as Structural Maintenance of Chromosomes Protein 2 is a critical component of the Condensin complex with a mass of approximately 135 kDa. This protein primarily functions within the nucleus of the cell where it plays an important role in chromosome condensation during cell division. SMC2 belongs to the SMC family of proteins and is widely expressed in various tissues including highly proliferative ones like bone marrow and thymus. The protein structure includes coiled-coil and hinge regions that are vital for its function in maintaining chromosome structure.
Biological function summary

SMC2 plays an essential role in ensuring proper chromosome segregation during mitosis. It acts as part of the Condensin complex a multi-protein assembly that is also composed of SMC4 CAP-D2 and CAP-G among others. This complex is important for the restructuring of chromosomes into compact forms allowing them to be easily separated during cell division. SMC2 binds to DNA and aids in the resolution of sister chromatids facilitating the equal distribution of genetic material to daughter cells.

Pathways

SMC2 operates within the cell cycle control pathway where it is involved in mitotic processes. It interacts directly with other components of the cell cycle machinery including Cyclin-dependent kinase 1 (CDK1). SMC2 also plays a role in the DNA repair pathway partnering with proteins like ATM (Ataxia-Telangiectasia Mutated kinase) which senses DNA damage and coordinates repair processes. These pathways highlight the interconnected nature of SMC2's functions in maintaining genetic stability.

SMC2 has been linked to certain cancers due to its pivotal role in ensuring proper mitosis. Disruptions in its function can lead to aneuploidy and genomic instability common features of tumorigenesis. Furthermore SMC2's involvement in DNA repair pathways implies a connection to disorders related to DNA damage like Ataxia-Telangiectasia. This disorder is marked by increased sensitivity to DNA damage and proteins like ATM are key partners of SMC2 in these repair processes. Understanding these links provides insights into potential therapeutic targets for addressing such diseases.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
See full target information SMC2
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文献 (22)

Recent publications for all applications. Explore the full list and refine your search

Genes & development 34:1619-1636 PubMed33122293

2020

Replication stress conferred by POT1 dysfunction promotes telomere relocalization to the nuclear pore.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra M Pinzaru,Mike Kareh,Noa Lamm,Eros Lazzerini-Denchi,Anthony J Cesare,Agnel Sfeir

Cell reports 31:107533 PubMed32320646

2020

Inducible Degradation of the Human SMC5/6 Complex Reveals an Essential Role Only during Interphase.

Applications

Unspecified application

Species

Unspecified reactive species

Andrés Bueno Venegas,Toyoaki Natsume,Masato Kanemaki,Ian D Hickson

Nucleus (Austin, Tex.) 11:1-18 PubMed31924112

2020

Hyperosmotic stress: chromatin phase separation.

Applications

Unspecified application

Species

Unspecified reactive species

Ada L Olins,Travis J Gould,Logan Boyd,Bettina Sarg,Donald E Olins

Journal of cell science 132: PubMed31653782

2019

Condensin II protein dysfunction impacts mitochondrial respiration and mitochondrial oxidative stress responses.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Deutschman,Jacqueline R Ward,Avinash Kumar,Greeshma Ray,Nicole Welch,Madeleine E Lemieux,Srinivisan Dasarathy,Michelle S Longworth

Nature cell biology 21:1393-1402 PubMed31685986

2019

A chromosome folding intermediate at the condensin-to-cohesin transition during telophase.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin Abramo,Anne-Laure Valton,Sergey V Venev,Hakan Ozadam,A Nicole Fox,Job Dekker

The Journal of cell biology 218:1531-1552 PubMed30858191

2019

Live imaging of marked chromosome regions reveals their dynamic resolution and compaction in mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

John K Eykelenboom,Marek Gierliński,Zuojun Yue,Nadia Hegarat,Hilary Pollard,Tatsuo Fukagawa,Helfrid Hochegger,Tomoyuki U Tanaka

Molecular biology of the cell 30:427-440 PubMed30586323

2018

Dissociation of membrane-chromatin contacts is required for proper chromosome segregation in mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lysie Champion,Sumit Pawar,Naemi Luithle,Rosemarie Ungricht,Ulrike Kutay

Cellular and molecular life sciences : CMLS 76:381-395 PubMed30368549

2018

NudCL2 is an Hsp90 cochaperone to regulate sister chromatid cohesion by stabilizing cohesin subunits.

Applications

Unspecified application

Species

Unspecified reactive species

Yuehong Yang,Wei Wang,Min Li,Ya Gao,Wen Zhang,Yuliang Huang,Wei Zhuo,Xiaoyi Yan,Wei Liu,Fangwei Wang,Dingwei Chen,Tianhua Zhou

Nature 561:551-555 PubMed30232450

2018

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Applications

Unspecified application

Species

Unspecified reactive species

Shiwei Liu,Mijung Kwon,Mark Mannino,Nachen Yang,Fioranna Renda,Alexey Khodjakov,David Pellman

Journal of cell science 131: PubMed29487178

2018

Ki-67 and condensins support the integrity of mitotic chromosomes through distinct mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Masatoshi Takagi,Takao Ono,Toyoaki Natsume,Chiyomi Sakamoto,Mitsuyoshi Nakao,Noriko Saitoh,Masato T Kanemaki,Tatsuya Hirano,Naoko Imamoto
View all publications
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保証に関する詳細については利用規約をご確認ください。
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