Anti-SMC2 抗体 (ab10399)
Key features and details
- Rabbit polyclonal to SMC2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SMC2 antibody
SMC2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SMC2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human SMC2 aa 50-150. The epitope maps to a region between residues 50 and 100 of human Structural Maintenance of Chromosomes 2 using the numbering given in entry NP_006435.1 (GeneID 10592).
Database link: O95347 -
ポジティブ･コントロール
- WB: Mock transfected or SMC2 transfected HEK-293T whole cell lysates. IHC-P: Human cerebral cortex tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antibodies were affinity purified using the peptide immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10399の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/5000 - 1/20000. Detects a band of approximately 150 kDa (predicted molecular weight: 143 kDa).
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/5000 - 1/20000. Detects a band of approximately 150 kDa (predicted molecular weight: 143 kDa). |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. -
配列類似性
Belongs to the SMC family. SMC2 subfamily. -
ドメイン
The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC4, forming a V-shaped heterodimer. -
細胞内局在
Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase. - Information by UniProt
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参照データベース
- Entrez Gene: 10592 Human
- Entrez Gene: 397800 Xenopus laevis
- Omim: 605576 Human
- SwissProt: O95347 Human
- SwissProt: P50533 Xenopus laevis
- Unigene: 119023 Human
- Unigene: 928 Xenopus laevis
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別名
- CAP E antibody
- CAPE antibody
- Chromosome associated protein E antibody
see all
画像
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Western blot - Anti-SMC2 antibody (ab10399)All lanes : Anti-SMC2 antibody (ab10399) at 0.1 µg/ml
Lane 1 : E: Mock transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : T: SMC2 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 90 µg per lane.
Predicted band size: 143 kDa
Exposure time: 10 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC2 antibody (ab10399)IHC image of ab10399 staining in normal human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10399, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab10399 は 8 報の論文で使用されています。
- Baergen AK et al. Diminished Condensin Gene Expression Drives Chromosome Instability That May Contribute to Colorectal Cancer Pathogenesis. Cancers (Basel) 11:N/A (2019). PubMed: 31357676
- Poser E et al. Aurora A promotes chromosome congression by activating the condensin-dependent pool of KIF4A. J Cell Biol 219:N/A (2019). PubMed: 31881080
- Shirali AS et al. A multi-step transcriptional cascade underlies vascular regeneration in vivo. Sci Rep 8:5430 (2018). PubMed: 29615716
- Wang D et al. Hydrogen gas inhibits lung cancer progression through targeting SMC3. Biomed Pharmacother 104:788-797 (2018). WB . PubMed: 29852353
- Gerasimova T et al. A structural hierarchy mediated by multiple nuclear factors establishes IgH locus conformation. Genes Dev 29:1683-95 (2015). ChIP ; Mouse . PubMed: 26302788
- Pan X et al. YY1 controls Ig? repertoire and B-cell development, and localizes with condensin on the Ig? locus. EMBO J 32:1168-82 (2013). PubMed: 23531880
- Hancock R Structure of metaphase chromosomes: a role for effects of macromolecular crowding. PLoS One 7:e36045 (2012). ICC/IF . PubMed: 22540018
- Fazzio TG & Panning B Condensin complexes regulate mitotic progression and interphase chromatin structure in embryonic stem cells. J Cell Biol 188:491-503 (2010). WB ; Mouse . PubMed: 20176923