Anti-SMC1A (phospho S966) 抗体 [EP2858Y] (ab81306)
![Western blot - Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)](https://www.abcam.co.jp/ps/products/81/ab81306/Images/ab81306-535043-anti-smc1a-phospho-s966-antibody-ep2858y-western-blot-human-hek-293.jpg "Western blot - Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2858Y] to SMC1A (phospho S966)
- Suitable for: Dot blot, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-SMC1A (phospho S966) antibody [EP2858Y]
SMC1A 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2858Y] to SMC1A (phospho S966) -
由来種
Rabbit -
アプリケーション
適用あり: Dot blot, WB, ICC/IFmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- WB: HEK-293 treated with 5mM Hydroxyurea. ICC/IF: HeLa cell lysate. Dot Blot: SMC1A (phospho S966) peptide.
-
特記事項
This product has switched from a hybridoma to recombinant production method on 23rd February 2024.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2858Y -
アイソタイプ
IgG -
研究分野
関連製品
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81306の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Dot blot |
1/1000.
|
|
| WB |
1/5000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).
|
|
| ICC/IF |
1/50.
|
| 特記事項 |
|---|
|
Dot blot
1/1000. |
|
WB
1/5000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa). |
|
ICC/IF
1/50. |
ターゲット情報
-
機能
Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint. -
関連疾患
Defects in SMC1A are the cause of Cornelia de Lange syndrome type 2 (CDLS2) [MIM:300590]; also known as Cornelia de Lange syndrome X-linked. CDLS is a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. CDLS is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation and various other malformations including gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies. -
配列類似性
Belongs to the SMC family. SMC1 subfamily. -
ドメイン
The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure. -
翻訳後修飾
Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation. -
細胞内局在
Nucleus. Chromosome. Chromosome > centromere > kinetochore. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis. - Information by UniProt
-
参照データベース
- Entrez Gene: 8243 Human
- Omim: 300040 Human
- SwissProt: Q14683 Human
- Unigene: 211602 Human
-
別名
- Chromosome segregation protein SmcB antibody
- DXS423E antibody
- KIAA0178 antibody
see all
画像
-
Western blot - Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)All lanes : Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306) at 1/5000 dilution
Lane 1 : Untreated HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HEK-293 treated with 5mM Hydroxyurea for 18h whole cell lysate
Lane 3 : HEK-293 treated with 5mM Hydroxyurea for 18h whole cell lysate, then the membrane was incubated with phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 143 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Exposure time: 40 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
-
![Immunocytochemistry/ Immunofluorescence - Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)](https://www.abcam.co.jp/ps/products/81/ab81306/Images/ab81306-535045-anti-smc1a-phospho-s966-antibody-ep2858y-immunocytochemistry-human-hela.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling SMC1A with ab81306 at 1/50 (2 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889) was used to counterstain tubulin at 1/200 dilution. Confocal image showing nuclear staining in HeLa cells treated with hydroxyurea(3mM) for 20h. The nuclear counterstain was DAPI (Blue).
-
![Dot Blot - Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)](https://www.abcam.co.jp/ps/products/81/ab81306/Images/ab81306-535047-anti-smc1a-phospho-s966-antibody-ep2858y-dot-blot-human.jpg)
Dot Blot - Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)Dot blot analysis of SMC1A (phospho S966) using ab81306 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1/100,000 dilution. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 180s.
Lane 1: SMC1A (phospho S966) peptide
Lane 2: SMC1A non-phospho peptide
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab81306 は論文での使用が確認できていません。