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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-SMARCD1 抗体 [EPR23170-71] (ab245222)

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Western blot - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
  • Immunocytochemistry/ Immunofluorescence - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
  • Immunoprecipitation - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
  • Flow Cytometry (Intracellular) - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
  • Western blot - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
  • Immunoprecipitation - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
  • Flow Cytometry (Intracellular) - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
  • Immunocytochemistry/ Immunofluorescence - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
  • Anti-SMARCD1 antibody [EPR23170-71] (ab245222)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23170-71] to SMARCD1
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-SMARCD1 antibody [EPR23170-71]
    SMARCD1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23170-71] to SMARCD1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IF, IPmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, 293T, NIH/3T3 and Neuro-2a lysates. ICC/IF: HeLa and Neuro-2a cells. Flow Cyt (intra): HeLa and NIH/3T3 cells. IP: HeLa and NIH/3T3 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245222の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
ICC/IF
1/50.
IP
1/30.
特記事項
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
ICC/IF
1/50.
IP
1/30.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Involved in chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Has a strong influence on the Vitamin D-mediated transcriptional activity from an enhancer Vitamin D receptor element (VDRE). May be a link between mammalian SWI-SNF-like chromatin remodeling complexes and the vitamin D receptor (VDR) heterodimer. Mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

  • 組織特異性

    Expressed in all tissues tested, including brain, heart, kidney, liver, lung, muscle, pancreas and placenta.

  • 配列類似性

    Belongs to the SMARCD family.
    Contains 1 SWIB domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 60 kDa BRG-1/Brm-associated factor subunit A antibody
      • 60 kDa BRG1/Brm associated factor subunit A antibody
      • BAF60A antibody
      • BRG1 associated factor 60A antibody
      • BRG1-associated factor 60A antibody
      • Chromatin remodeling complex BAF60A subunit antibody
      • CRACD1 antibody
      • Mammalian chromatin remodeling complex BRG1 associated factor 60A antibody
      • Rsc6p antibody
      • SMARCD1 antibody
      • SMRD1 antibody
      • SMRD1_HUMAN antibody
      • SWI/SNF complex 60 kDa subunit A antibody
      • SWI/SNF complex 60 kDa subunit antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin d1 antibody
      • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 antibody
      • Swp73 like protein antibody
      see all

    画像

    • Western blot - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
      Western blot - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
      All lanes : Anti-SMARCD1 antibody [EPR23170-71] (ab245222) at 1/1000 dilution

      Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : SMARCD1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 58 kDa
      Observed band size: 58 kDa



      Lanes 1-4: Merged signal (red and green). Green - ab245222 observed at 58 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab245222 Anti-SMARCD1 antibody [EPR23170-71] was shown to specifically react with SMARCD1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266458 (knockout cell lysate ab259143) was used. Wild-type and SMARCD1 knockout samples were subjected to SDS-PAGE. ab245222 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
      Immunocytochemistry/ Immunofluorescence - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling SMARCD1 with ab245222 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in Neuro-2a cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    • Immunoprecipitation - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
      Immunoprecipitation - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)

      SMARCD1 was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate with ab245222 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245222 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

      Lane 1: NIH/3T3 whole cell lysate 10 (Input).
      Lane 2: ab245222 IP in NIH/3T3 whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245222 in NIH/3T3 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 3 minutes.

    • Flow Cytometry (Intracellular) - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
      Flow Cytometry (Intracellular) - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling SMARCD1 with ab245222 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Western blot - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
      Western blot - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
      All lanes : Anti-SMARCD1 antibody [EPR23170-71] (ab245222) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
      Lane 2 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
      Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg
      Lane 4 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 58 kDa
      Observed band size: 58 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • Immunoprecipitation - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
      Immunoprecipitation - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)

      SMARCD1 was immunoprecipitated from 0.35 mg of HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab245222 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245222 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

      Lane 1: HeLa whole cell lysate 10 (Input). 
      Lane 2: ab245222 IP in HeLa whole cell lysate. 
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245222 in HeLa whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 3 minutes.

    • Flow Cytometry (Intracellular) - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
      Flow Cytometry (Intracellular) - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling SMARCD1 with ab245222 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
      Immunocytochemistry/ Immunofluorescence - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling SMARCD1 with ab245222 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    • Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
      Anti-SMARCD1 antibody [EPR23170-71] (ab245222)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab245222 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab245222 は論文での使用が確認できていません。

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