Anti-SmarcAL1 抗体 (ab154226)
Key features and details
- Rabbit polyclonal to SmarcAL1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-SmarcAL1 antibody
SmarcAL1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SmarcAL1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to a region within amino acids 120 and 457 of Human SmarcAL1 (UniProt ID: Q9NZC9)
-
ポジティブ･コントロール
- A549 whole cell lysate, A549 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154226の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 106 kDa.
|
| ICC/IF |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 106 kDa. |
|
ICC/IF
1/100 - 1/1000. |
ターゲット情報
-
機能
ATP-dependent annealing helicase that catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. -
組織特異性
Ubiquitously expressed, with high levels in testis. -
関連疾患
Defects in SMARCAL1 are a cause of Schimke immuno-osseous dysplasia (SIOD) [MIM:242900]. SIOD causes spondyloepiphyseal dysplasia, renal dysfunction and T-cell immunodeficiency. Approximately half of all patients also exhibit hyperthyroidism, while around half also exhibit episodal cerebral ischema. -
配列類似性
Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.
Contains 2 HARP domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 50485 Human
- Omim: 606622 Human
- SwissProt: Q9NZC9 Human
- Unigene: 516674 Human
-
別名
- HARP antibody
- HepA Related Protein antibody
- HepA-related protein antibody
see all
画像
-
Western blot - Anti-SmarcAL1 antibody (ab154226)Anti-SmarcAL1 antibody (ab154226) at 1/500 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 106 kDa
5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-SmarcAL1 antibody (ab154226)Immunofluorescence analysis of paraformaldehyde-fixed A549 cells, labeling SmarcAL1 with ab154226 at a 1/500 dilution (top image), and costained with Hoescht 33342 (bottom image).
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab154226 は 3 報の論文で使用されています。
- Hambarde S et al. EXO5-DNA structure and BLM interactions direct DNA resection critical for ATR-dependent replication restart. Mol Cell 81:2989-3006.e9 (2021). PubMed: 34197737
- Pugliese GM et al. Inducible SMARCAL1 knockdown in iPSC reveals a link between replication stress and altered expression of master differentiation genes. Dis Model Mech 12:N/A (2019). PubMed: 31515241
- Keka IS et al. Smarcal1 promotes double-strand-break repair by nonhomologous end-joining. Nucleic Acids Res N/A:N/A (2015). Human . PubMed: 26089390