Anti-SMAD9 抗体 (ab48011)
Key features and details
- Goat polyclonal to SMAD9
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SMAD9 antibody
SMAD9 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to SMAD9 -
由来種
Goat -
特異性
Not expected to crossreact with SMAD1 or SMAD5. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Dog
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免疫原
Synthetic peptide:
C-HSEPLMPHNATYPD
, corresponding to internal sequence amino acids 166-179 of Human SMAD9. -
ポジティブ･コントロール
- Human skin lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab48011の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD). -
組織特異性
Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney. -
関連疾患
Pulmonary hypertension, primary, 2 -
配列類似性
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
翻訳後修飾
Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. -
細胞内局在
Cytoplasm. Nucleus. In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4. - Information by UniProt
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参照データベース
- Entrez Gene: 4093 Human
- Entrez Gene: 55994 Mouse
- Entrez Gene: 85435 Rat
- Omim: 603295 Human
- SwissProt: O15198 Human
- SwissProt: Q9JIW5 Mouse
- SwissProt: O54835 Rat
- Unigene: 123119 Human
see all -
別名
- MAD homolog 9 antibody
- Madh6 antibody
- Mothers against decapentaplegic antibody
see all
画像
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Western blot - Anti-SMAD9 antibody (ab48011)Anti-SMAD9 antibody (ab48011) at 0.3 µg/ml + Human Skin lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 52 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab48011 は 1 報の論文で使用されています。
- Katakawa Y et al. Smad8/9 Is Regulated Through the BMP Pathway. J Cell Biochem 117:1788-96 (2016). PubMed: 26748560