AB51177

Anti-Smad3 (phospho S425) 抗体

Name: Anti-Smad3 (phospho S425) 抗体 (ab51177) | Abcam
Brand: Abcam Limited
SKU: AB51177
Price: 78000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-Smad3 (phospho S425) antibody

(1 Review)

(20 Publications)

Rabbit Polyclonal SMAD3 phospho S425 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD3 phospho S425 aa 350 to C-terminus.

別名を表示する

MADH3, SMAD3, SMAD family member 3, SMAD 3, hSMAD3, JV15-2, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad3 (phospho S425) antibody (AB51177)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad3 (phospho S425) antibody (AB51177)

ab47439, at dilution of 1/50, staining Smad3 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image : No peptide.
Right image : Phosphopeptide present.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P

applications

免疫原

Synthetic Peptide within Human SMAD3 phospho S425 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P84022

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Smad3 also known as Mothers against decapentaplegic homolog 3 is a protein that plays a mechanical role in signal transduction. It acts mainly as a transcription factor and gets activated through phosphorylation. The molecular weight of Smad3 is approximately 48 kDa. It is expressed widely across numerous tissues including the cellular nucleus where it executes its function after activation.

Biological function summary

Smad3 acts as a mediator of signal transduction for the TGF-beta (transforming growth factor-beta) superfamily forming a complex with phosphorylated Smad2. This enables it to regulate transcriptional activity influencing cell proliferation differentiation and apoptosis. Smad3 also participates in various cellular processes by interacting with other co-factors and regulatory proteins that aid in fine-tuning its function.

Pathways

Smad3 plays an important role in the TGF-beta signaling pathway where it works closely with Smad4 to propagate the signal. Upon phosphorylation it forms a complex with co-Smad (Smad4) and moves into the nucleus to influence gene expression. Smad3 is also involved in pathways related to oncogenesis and tissue fibrosis indicating its significant role in cellular regulation and response mechanisms.

Smad3 is associated with fibrotic diseases and cancers particularly in tissues such as the liver and lungs. Altered Smad3 signaling contributes to the pathological process occurring in fibrotic disorders often interacting with Smad4 in these abnormalities. Dysregulated Smad3 expression or mutations can also lead to oncogenic transformations highlighting its critical involvement in disease states.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

See full target information SMAD3 phospho S425

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文献 (20)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular biology (Noisy-le-Grand, France) 70:95-101 PubMed38650149

2024

Ginsenoside Rc alleviates osteoporosis by the TGF-β/Smad signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shanfu Wang,Bing Xu,Heng Yin,Zhen Hua,Yang Shao,Jianwei Wang

The International journal of developmental biology 64:423-432 PubMed33063836

2020

Control of fibrosis by TGFβ signalling modulation promotes redifferentiation during limited regeneration of mouse ear.

Applications

Unspecified application

Species

Unspecified reactive species

RenéFernando Abarca-Buis,María Elena Contreras-Figueroa,David Garciadiego-Cázares,Edgar Krötzsch

Scientific reports 10:5118 PubMed32198404

2020

Tenascin-C inactivation impacts lung structure and function beyond lung development.

Applications

Unspecified application

Species

Unspecified reactive species

Sandrine Gremlich,Matthias Roth-Kleiner,Lucile Equey,Kleanthis Fytianos,Johannes C Schittny,Tiziana P Cremona

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 35:167-180 PubMed31487395

2019

High-Dose TGF-β1 Impairs Mesenchymal Stem Cell-Mediated Bone Regeneration via Bmp2 Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Xu,Jinlong Liu,Yaokai Gan,Kerong Dai,Jingyu Zhao,Mingjian Huang,Yan Huang,Yifu Zhuang,Xiaoling Zhang

The Journal of pathology 249:26-38 PubMed30953361

2019

Prosaposin is a biomarker of mesenchymal glioblastoma and regulates mesenchymal transition through the TGF-β1/Smad signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Jiang,Jinpeng Zhou,Dianqi Hou,Peng Luo,Huiling Gao,Yanju Ma,Yin-Sheng Chen,Long Li,Dan Zou,Haiying Zhang,Ye Zhang,Zhitao Jing

American journal of translational research 11:1116-1128 PubMed30899411

2019

PDCD5 inhibits osteosarcoma cell metastasis via targeting TGF-β1/Smad signaling pathway and is associated with good prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhao,Changliang Peng,Xinchang Lu,Meng Guo,Tiejun Yang,Junlin Zhou,Yong Hai

Cellular physiology and biochemistry : internation 44:2201-2211 PubMed29253845

2017

Plasminogen Activator Inhibitor 1 Promotes Immunosuppression in Human Non-Small Cell Lung Cancers by Enhancing TGF-Β1 Expression in Macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Chengjun Zhu,Hua Shen,Lingjun Zhu,Feng Zhao,Yongqian Shu

Journal of experimental & clinical cancer research 36:141 PubMed29020987

2017

SHCBP1 promotes synovial sarcoma cell metastasis via targeting TGF-β1/Smad signaling pathway and is associated with poor prognosis.

Applications

IHC

Species

Human

Changliang Peng,Hui Zhao,Yan Song,Wei Chen,Xiaoying Wang,Xiaoli Liu,Cheng Zhang,Jie Zhao,Ji Li,Guanghui Cheng,Dongjin Wu,Chunzheng Gao,Xiuwen Wang

The Journal of biological chemistry 292:19423-19440 PubMed28982980

2017

Inhibition of the transforming growth factor-β/SMAD cascade mitigates the anti-neurogenic effects of the carbamate pesticide carbofuran.

Applications

Unspecified application

Species

Unspecified reactive species

Brashket Seth,Anuradha Yadav,Swati Agarwal,Shashi Kant Tiwari,Rajnish Kumar Chaturvedi

International journal of molecular medicine 40:1965-1970 PubMed29039466

2017

RACK1 silencing attenuates renal fibrosis by inhibiting TGF-β signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Feng,Liyi Xie,Ranran Kong,Yali Zhang,Kehui Shi,Wanhong Lu,Hongli Jiang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Anti-Smad3 (phospho S425) 抗体

Anti-Smad3 (phospho S425) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad3 (phospho S425) antibody (AB51177)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (20)

代替製品

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