Anti-Smad2 (phospho S467) 抗体 (ab53100)

I can confirm that PMA is indeed Phorbol 12-myristate 13-acetate and it was used to induce the modification of Phospho-Smad2 at S467 site.

I am sorry to hear that this product has not been performing as stated on the datasheet and for the inconvenience that this has caused. This product is highly modified post translationally. Have you performed these blots under reducing conditions? This product is covered by our Abpromise guarantee. We are happy provide scientific support at any time. If you are using the product in species and applications listed on the datasheet and contact us within six months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue. More information on our Abpromise may be found at the following link: https://www.abcam.com/index.html?pageconfig=abpromise

Thank you very much for your reply. My colleague is out of the office this week, but I'll be happy to help you with this enquiry.

You could use ab53100 in a sandwich ELISA to study the SMAD2 protein phosphorylated at residue S467. I'd suggest using this as the detection antibody and a total SMAD2 antibody as the capture antibody. Ab47083 is a total SMAD2 antibody that has been tested for reactivity with bovine samples, so this may be a good choice-

https://www.abcam.com/smad2-antibody-ab47083.html

Since this is also a rabbit polyclonal antibody, you would need to directly conjugate the detection antibody to HRP or AP because an anti-rabbit secondary antibody will bind to both of the primary antibodies. We have conjugation kits which can be used for this purpose-

https://www.abcam.com/easylink-hrp-conjugation-kit-3-x-100µg-hrp-ab102891.html

Another option would be to use a mouse monoclonal total SMAD2 antibody that hasn't been tested with bovine samples, since the protein is believed to be fairly conserved and it might cross-react with your samples. In this case, you could use a pre-adsorbed anti-rabbit secondary antibody like ab7090 that won't cross-react with the mouse capture IgG. We have ab119907 which could be used for this-

https://www.abcam.com/smad2-antibody-5g7-ab119907.html

Since we haven't tested ab119907 or ab53100 with bovine samples, we can't guarantee that they will cross-react and work in your assay. However we do have a testing discount program that you can use to try these antibodies. As part of our AbTrial program, if you submit your protocol and results to us as an online Abreview, we will give you a discount on a future purchase worth the purchase price of ab119907 and/or ab53100. I'd be happy to send more information about this program if you would like.

I hope that this will be helpful, but if you have further questions or need anything else, please let me know and I'll be happy to assist you further. Have a nice day.