Anti-Smad2 (phospho S250) 抗体 [EPR2855(2)] (ab184557)
![Western blot - Anti-Smad2 (phospho S250) antibody [EPR2855(2)] (ab184557)](https://www.abcam.co.jp/ps/products/184/ab184557/Images/ab184557-216795-anti-smad2-phospho-s250-antibody-epr28552-western-blot.jpg "Western blot - Anti-Smad2 (phospho S250) antibody [EPR2855(2)] (ab184557)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2855(2)] to Smad2 (phospho S250)
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Smad2 (phospho S250) antibody [EPR2855(2)]
Smad2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2855(2)] to Smad2 (phospho S250) -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HeLa cell lysate treated with alkaline phosphatase; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2855(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184557の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa). |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. -
組織特異性
Expressed at high levels in skeletal muscle, heart and placenta. -
配列類似性
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
翻訳後修飾
Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. - Information by UniProt
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参照データベース
- Entrez Gene: 4087 Human
- Entrez Gene: 17126 Mouse
- Entrez Gene: 29357 Rat
- Omim: 601366 Human
- SwissProt: Q15796 Human
- SwissProt: Q62432 Mouse
- SwissProt: O70436 Rat
- Unigene: 12253 Human
see all -
別名
- Drosophila, homolog of, MADR2 antibody
- hMAD-2 antibody
- HsMAD2 antibody
see all
画像
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Western blot - Anti-Smad2 (phospho S250) antibody [EPR2855(2)] (ab184557)All lanes : Anti-Smad2 (phospho S250) antibody [EPR2855(2)] (ab184557) at 1/1000 dilution
Lane 1 : untreated HeLa cells lysates
Lane 2 : HeLa cell lysate treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-Smad2 (phospho S250) antibody [EPR2855(2)] (ab184557)](https://www.abcam.co.jp/ps/products/184/ab184557/Images/ab184557-216797-anti-smad2-phospho-s250-antibody-epr28552-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Smad2 (phospho S250) antibody [EPR2855(2)] (ab184557)Intracellular flow cytometric analysis of2% paraformaldehyde-fixed and permeabilized HeLa cells labeling Smad2 (phospho S250) with ab184557 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. HeLa cells labeled using ab184557 preincubated with Smad2 (phospho S250) peptide (blue) or non-phospho-Smad2 peptide (orange).
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![Anti-Smad2 (phospho S250) antibody [EPR2855(2)] (ab184557)](https://www.abcam.co.jp/ps/products/184/ab184557/Images/ab184557-3-benefits-of-recombinant-antibodies.png)
Anti-Smad2 (phospho S250) antibody [EPR2855(2)] (ab184557)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (6)
ab184557 は 6 報の論文で使用されています。
- Zheng YC et al. Islet transplantation ameliorates diabetes-induced testicular interstitial fibrosis and is associated with inhibition of TGF-ß1/Smad2 pathway in a rat model of type 1 diabetes. Mol Med Rep 23:N/A (2021). PubMed: 33760134
- Qu S et al. EZH2 is involved in psoriasis progression by impairing miR-125a-5p inhibition of SFMBT1 and leading to inhibition of the TGFß/SMAD pathway. Ther Adv Chronic Dis 12:2040622320987348 (2021). PubMed: 33948156
- Sun M et al. MicroRNA-302b mitigates renal fibrosis via inhibiting TGF-ß/Smad pathway activation. Braz J Med Biol Res 54:e9206 (2021). PubMed: 33503202
- Zhou T et al. GDF10 inhibits proliferation and epithelial-mesenchymal transition in triple-negative breast cancer via upregulation of Smad7. Aging (Albany NY) 11:3298-3314 (2019). PubMed: 31147529
- Amer S et al. Role of TGF-ß in the motility of ShcD-overexpressing 293 cells. Mol Med Rep 20:2667-2674 (2019). PubMed: 31524262
- Li Y et al. LPS induces HUVEC angiogenesis in vitro through miR-146a-mediated TGF-ß1 inhibition. Am J Transl Res 9:591-600 (2017). WB ; Human . PubMed: 28337286