Anti-Smad2 抗体 (ab63576)
Key features and details
- Rabbit polyclonal to Smad2
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Smad2 antibody
Smad2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Smad2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic non-phosphopeptide derived from human Smad2 around the phosphorylation site of serine 467 (C-S-S-M-S).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63576の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (2) |
1/50 - 1/100.
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| ICC/IF | (1) |
1/500 - 1/1000.
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| 特記事項 |
|---|
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IHC-P
1/50 - 1/100. |
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ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. -
組織特異性
Expressed at high levels in skeletal muscle, heart and placenta. -
配列類似性
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
翻訳後修飾
Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. - Information by UniProt
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参照データベース
- Entrez Gene: 4087 Human
- Omim: 601366 Human
- SwissProt: Q15796 Human
- Unigene: 12253 Human
- Unigene: 705764 Human
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別名
- Drosophila, homolog of, MADR2 antibody
- hMAD-2 antibody
- HsMAD2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 antibody (ab63576)ab63576, at a 1/50 dilution, staining human Smad2 in prostate carcinoma, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue, minus immunising peptide (left image) and plus immunising peptide (right image).
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Immunocytochemistry/ Immunofluorescence - Anti-Smad2 antibody (ab63576)Immunofluorescent staining of HepG2 cells, using ab63576 at a 1/500 dilution, minus immunising peptide (left image) and plus immunising peptide (right image). -
Immunocytochemistry/ Immunofluorescence - Anti-Smad2 antibody (ab63576)This image is courtesy of an anonymous Abreviewab63576 staining Smad2 in Mouse enterocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton and blocked with 5% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/250) for 2 hours at 20°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (26)
ab63576 は 26 報の論文で使用されています。
- He T et al. Tetrahydrocurcumin (THC) enhanced the clearance of Cryptococcus deneoformans during infection in vivo. Antonie Van Leeuwenhoek 116:565-576 (2023). PubMed: 37186068
- Dhakal P et al. Circulating activin A during equine gestation and immunolocalization of its receptors system in utero-placental tissues and fetal gonads. J Equine Sci 32:39-48 (2021). PubMed: 34220270
- Bourne MH et al. Vardenafil Activity in Lung Fibrosis and In Vitro Synergy with Nintedanib. Cells 10:N/A (2021). PubMed: 34944010
- Qin W et al. Spatiotemporally-resolved mapping of RNA binding proteins via functional proximity labeling reveals a mitochondrial mRNA anchor promoting stress recovery. Nat Commun 12:4980 (2021). PubMed: 34404792
- Wang W et al. Promotion of chemerin in rat diabetic kidney disease through enhancement of TGF-ß1/Smads/CTGF pathway. Am J Transl Res 13:10206-10217 (2021). PubMed: 34650691