Anti-SMAD1/5/9 抗体 [EPR25803-154] (BSA and Azide free) (ab300165)
![Western blot - Anti-SMAD1/5/9 antibody [EPR25803-154] (BSA and Azide free) (AB300165)](https://www.abcam.co.jp/ps/products/300/ab300165/Images/AB300165-489232-anti-smad159-antibody-epr25803-154-western-blot-2.jpg "Western blot - Anti-SMAD1/5/9 antibody [EPR25803-154] (BSA and Azide free) (AB300165)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25803-154] to SMAD1/5/9 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SMAD1/5/9 antibody [EPR25803-154] (BSA and Azide free)
SMAD1/5/9 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR25803-154] to SMAD1/5/9 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt (Intra),ICC/IF or IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers. -
ポジティブ･コントロール
- WB: Whole cell lysates: HeLa, 293T, NIH/3T3, PC-12, THP-1, Jurkat, HEK-293T cells transfected with a His-tagged human SMAD1 or SMAD5 or SMAD9 expression vector, HeLa transfected with scrambled siRNA control, HeLa transfected with siRNA specifically targeting SMAD9.
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特記事項
Ab300165 is a carrier free version of ab300164.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25803-154 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300165の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 62 kDa.
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| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 62 kDa. |
ターゲット情報
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関連性
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs, common-mediator SMAD and inhibitory SMADs. SMAD1, SMAD5 and SMAD9 have high degrees of homology. SMAD8 and SMAD9 are used as alternate names for the same protein. Human SMAD1 is a 465 amino acid protein, human SMAD5 is a 465 amino acid and human SMAD9 is a 430 amino acid protein. -
細胞内局在
Cytoplasmic and Nuclear -
参照データベース
- Entrez Gene: 4086 Human
- Entrez Gene: 4090 Human
- Entrez Gene: 4093 Human
- Entrez Gene: 17125 Mouse
- Entrez Gene: 17129 Mouse
- Entrez Gene: 55994 Mouse
- Entrez Gene: 25671 Rat
- Entrez Gene: 59328 Rat
see all -
別名
- AI528653 antibody
- BSP1 antibody
- DKFZp781C1895 antibody
see all
画像
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Western blot - Anti-SMAD1/5/9 antibody [EPR25803-154] (BSA and Azide free) (AB300165)All lanes : Anti-SMAD1/5/9 antibody [EPR25803-154] (ab300164) at 1/1000 dilution
Lane 1 : HEK-293T cells transfected with a human SMAD1 expression vector containing a his-tag, whole cell lysate at 10 µg
Lane 2 : HEK-293T cells transfected with a human SMAD2 expression vector containing a his-tag, whole cell lysate at 20 µg
Lane 3 : HEK-293T cells transfected with a human SMAD3 expression vector containing a his-tag, whole cell lysate at 20 µg
Lane 4 : HEK-293T cells transfected with a human SMAD5 expression vector containing a his-tag, whole cell lysate at 10 µg
Lane 5 : His-tagged human SMAD6 recombinant protein, 10 ng
Lane 6 : HEK-293T cells transfected with a human SMAD7 expression vector containing a his-tag, whole cell lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 20-62 kDa why is the actual band size different from the predicted?This data was developed using ab300164, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 7.75 seconds.
This antibody reacts with human SMAD1, SMAD5, and SMAD9, but does not cross-react with human SMAD2, SMAD3, SMAD6, and SMAD7.
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![Western blot - Anti-SMAD1/5/9 antibody [EPR25803-154] (BSA and Azide free) (AB300165)](https://www.abcam.co.jp/ps/products/300/ab300165/Images/AB300165-489233-anti-smad159-antibody-epr25803-154-western-blot-1.jpg)
Western blot - Anti-SMAD1/5/9 antibody [EPR25803-154] (BSA and Azide free) (AB300165)All lanes : Anti-SMAD1/5/9 antibody [EPR25803-154] (ab300164) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 5 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 6 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 147 secondsThis data was developed using ab300164, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 21220940).
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
The identity of the band at approximately 37 kDa is unknown.
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![Western blot - Anti-SMAD1/5/9 antibody [EPR25803-154] (BSA and Azide free) (AB300165)](https://www.abcam.co.jp/ps/products/300/ab300165/Images/AB300165-489235-anti-smad159-antibody-epr25803-154-western-blot-hela.jpg)
Western blot - Anti-SMAD1/5/9 antibody [EPR25803-154] (BSA and Azide free) (AB300165)All lanes : Anti-SMAD1/5/9 antibody [EPR25803-154] (ab300164) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate
Lane 2 : HeLa transfected with siRNA specifically targeting SMAD9, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab300164, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 21220940).
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
The identity of the band at approximately 37 kDa is unknown.
-
![Anti-SMAD1/5/9 antibody [EPR25803-154] (BSA and Azide free) (AB300165)](https://www.abcam.co.jp/ps/products/300/ab300165/Images/AB300165-489227-Benefits-of-Recombinant-antibodies.jpg)
Anti-SMAD1/5/9 antibody [EPR25803-154] (BSA and Azide free) (AB300165)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300165 は論文での使用が確認できていません。
