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Anti-SMAD1/5/9 抗体 (ab80255)

Reviews (3)Q&A (2)References (13)
Western blot - Anti-SMAD1/5/9 antibody (ab80255)

    Key features and details

    • Rabbit polyclonal to SMAD1/5/9
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] (ab108965)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-SMAD1/5/9 antibody
      SMAD1/5/9 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to SMAD1/5/9

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse

    • 免疫原

      Synthetic peptide derived from N terminal of human SMAD1/5/9

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab80255の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (2)
    1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
    特記事項
    WB
    1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

    ターゲット情報

    • 関連性

      SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs, common-mediator SMAD and inhibitory SMADs. SMAD1, SMAD5 and SMAD9 have high degrees of homology. SMAD8 and SMAD9 are used as alternate names for the same protein. Human SMAD1 is a 465 amino acid protein, human SMAD5 is a 465 amino acid and human SMAD9 is a 430 amino acid protein.

    • 細胞内局在

      Cytoplasmic and Nuclear

    • 参照データベース

      see all

    • 別名

      • AI528653 antibody
      • BSP1 antibody
      • DKFZp781C1895 antibody
      • DKFZp781O1323 antibody
      • Dwarfin A antibody
      • Dwf A antibody
      • Dwfc antibody
      • hSMAD1 antibody
      • hSmad5 antibody
      • JV4 1 antibody
      • JV41 antibody
      • JV5 1 antibody
      • MAD (mothers against decapentaplegic, Drosophila) homolog 1 antibody
      • MAD (mothers against decapentaplegic, Drosophila) homolog 9 antibody
      • MAD homolog 1 antibody
      • MAD homolog 9 antibody
      • MAD homolog1 (mothers against decapentaplegic, Drosophila) antibody
      • Mad related protein 1 antibody
      • MAD, mothers against decapentaplegic homolog 9 antibody
      • Madh1 antibody
      • MADH5 antibody
      • MADH6 antibody
      • MADH9 antibody
      • MADR1 antibody
      • Mlp1 antibody
      • Mothers against decapentaplegic, drosophila, homolog of, 9 antibody
      • Mothers against DPP homolog 1 antibody
      • Mothers against DPP homolog 9 antibody
      • MusMLP antibody
      • OTTHUMP00000178565 antibody
      • OTTMUSP00000022970 antibody
      • OTTMUSP00000022971 antibody
      • OTTMUSP00000022972 antibody
      • OTTMUSP0000002324 antibody
      • OTTMUSP00000023428 antibody
      • Sma- and Mad-related protein 1 antibody
      • SMAD antibody
      • SMAD family member 5 antibody
      • SMAD8 antibody
      • SMAD8A antibody
      • SMAD8B antibody
      • TGF beta signaling protein 1 antibody
      • Transforming growth factor-beta signaling protein 1 antibody
      see all

    画像

    • Western blot - Anti-SMAD1/5/9 antibody (ab80255)
      Western blot - Anti-SMAD1/5/9 antibody (ab80255)
      All lanes : Anti-SMAD1/5/9 antibody (ab80255) at 1/500 dilution

      Lane 1 : HeLa cell extracts
      Lane 2 : HeLa cell extracts with immunising peptide at 10 µg

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 52 kDa
      Observed band size: 52 kDa

    参考文献 (13)

    ab80255 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab80255 は 13 報の論文で使用されています。

    • Chen H  et al. Secreted phosphoprotein 24 kD (Spp24) inhibits the growth of human osteosarcoma through the BMP-2/Smad signaling pathway. J Orthop Res 41:1803-1814 (2023). PubMed: 36883270
    • Kabwe JC  et al. CRISPR-mediated Bmpr2 point mutation exacerbates late pulmonary vasculopathy and reduces survival in rats with experimental pulmonary hypertension. Respir Res 23:87 (2022). PubMed: 35395852
    • Kim JH  et al. Albiflorin Promotes Osteoblast Differentiation and Healing of Rat Femoral Fractures Through Enhancing BMP-2/Smad and Wnt/β-Catenin Signaling. Front Pharmacol 12:690113 (2021). PubMed: 34349649
    • Nakajima T  et al. Changes in Smad1/5/9 expression and phosphorylation in astrocytes of the rat hippocampus after transient global cerebral ischemia. J Chem Neuroanat 113:101941 (2021). PubMed: 33711423
    • Jin T  et al. CHI3L1 promotes Staphylococcus aureus-induced osteomyelitis by activating p38/MAPK and Smad signaling pathways. Exp Cell Res 403:112596 (2021). PubMed: 33826950
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-SMAD1/5/9 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (chondrocytes)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    chondrocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 12 2018

    Western blot abreview for Anti-SMAD1/5/9 antibody

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (embryonic stem cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    embryonic stem cells
    Blocking step
    Odyssey blocking buffer, LiCor as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Nov 01 2016

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SMAD1/5/9 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Cell (Rat cortex (day P7))
    Specification
    Rat cortex (day P7)
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Ruma Raha-Chowdhury

    Verified customer

    投稿 Dec 21 2009

    Question

    Can you please send me a list of the antibodies you have available for total SMAD 1/5 and phospho S463 / S465? I am looking for two antibodies to use in WB on the LICOR Odyssey system, so they should be raised in different species.

    Read More
    Answer

    Thank you for contacting us.

    We currently have two rabbit polyclonal antibodies that will detect SMAD 1/5:

    Rabbit polyclonal to SMAD1/5/9 - ab80255
    Rabbit polyclonal to SMAD 1/5/8/9 - ab13723

    We have a number of antibodies that will detect the S463 or S465 phosphorylation on SMAD 1 or SMAD 5:

    Rabbit polyclonal to Smad1 (phospho S465)- ab63439
    Rabbit monoclonal [EP728(2)AY] to SMAD5 (phospho S463 + S465)- ab76296
    Rabbit monoclonal [MMC-1-104-3] to SMAD5 (phospho S463 + S465) - ab92698
    Rabbit polyclonal to Smad1 (phospho S465)- ab97689
    Rabbit polyclonal to Smad1 (phospho S465)- ab97716

    While we only currently carry antibodies raised in rabbit for these targets, you could use our EasyLink conjugation kits to conjugate these antibodies to Cy5.5 or Cy7. These two fluorophores should be compatible with the Odyssey system.

    https://www.abcam.com/EasyLink

    Read More

    Question

    Are these antibodies capable of capturing native, endogenous SMAD1?

    Read More
    Answer

    Thank you for contacting us. Of the three antibodies we discussed yesterday, ab80255 is the best choice, given that we know the immunogen is derived from the N terminus, and your detection antibody is directed against the C terminus. The epitope that ab75273 binds to is unmapped, and so I am concerned that it may not be compatible in a sandwich ELISA with your detection antibody if, by chance, it binds to the C terminus. Antibody ab72504 was actually a duplicate entry of ab80255 and has been removed from the catalogue. Ab80255 has not, however, been tested in ELISA to capture native SMAD1. As I expected, it was validated for ELISA against the peptide that was used as the immunogen. It has been validated for IHC, though, so we expect it will be capable of binding the N terminus when SMAD1 is in its native conformation. Another antibody to consider is ab63356. The situation is the same as for ab80255: untested in ELISA against native, endogenous SMAD1, only against the peptide, but it gives a signal in IHC and ICC. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

    Read More

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