Anti-SMAD1/3/5 (phospho S423 + S425) 抗体 (ab51451)
Key features and details
- Rabbit polyclonal to SMAD1/3/5 (phospho S423 + S425)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SMAD1/3/5 (phospho S423 + S425) antibody -
製品の詳細
Rabbit polyclonal to SMAD1/3/5 (phospho S423 + S425) -
由来種
Rabbit -
特異性
ab51451 is specific for the phosphorylated form of the protein although minimal reactivity with nonphosphorylated human Smad3 can be observed by ELISA and WB. ab51451 also reacts with phosphorylated Smad1 and Smad5 but negligible reactivity is seen against other phosphorylated Smad family members.
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アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Pig, Xenopus laevis, Zebrafish
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免疫原
Synthetic peptide corresponding to Human SMAD1/3/5 aa 400-500 (phospho S423 + S425).
Database link: P84022 -
ポジティブ･コントロール
- WB: HEK293, HeLa, MCF-7, Jurkat, A431, A549, LNCap, MOLT-4, Ramos, Raji, A-172, NIH/3T3 Lysate; MEF lysate; Hep3B lysat.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51451の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (6) |
1/500.
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| WB | (3) |
1/2000 - 1/20000. Predicted molecular weight: 48 kDa. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested.
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| 特記事項 |
|---|
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IHC-P
1/500. |
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WB
1/2000 - 1/20000. Predicted molecular weight: 48 kDa. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested. |
ターゲット情報
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参照データベース
- SwissProt: P84022 Human
- SwissProt: Q15797 Human
- SwissProt: Q99717 Human
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別名
- BSP-1 antibody
- BSP1 antibody
- DKFZP586N0721 antibody
see all
画像
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Western blot - Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451)All lanes : Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451) at 1 µg/ml
Lane 1 : HEK293 lysate
Lane 2 : HeLa Lysate
Lane 3 : MCF-7 Lysate
Lane 4 : Jurkat Lysate
Lane 5 : A431 Lysate
Lane 6 : A549 Lysate
Lane 7 : LNCap Lysate
Lane 8 : MOLT-4 Lysate
Lane 9 : Ramos Lysate
Lane 10 : Raji Lysate
Lane 11 : A-172 Lysate
Lane 12 : NIH/3T3 Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 & 4-12 : Peroxidase rabbit secondary antibody at 1/30000 dilution
Lane 3 : Peroxidase rabbit secondary antibody
Predicted band size: 48 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451)ab51451 at 2.5 µg/ml staining epidermal keratinocytes at 40X. This image shows strong nuclear staining with localization of the antibody as the precipated red siganl and a hematoxylin purple nuclear counterstain.
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Western blot - Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451)All lanes : Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451) at 1/2000 dilution
Lane 1 : MEF lysate (control)
Lane 2 : MEF lysate, after stimulation with 2 ng/ml of TGF-ß for 1 hour
Lane 3 : MEF-/-Smad3 lysate (control)
Lane 4 : MEF-/-Smad3 lysate, after stimulation with 2 ng/ml of TGF-ß for one hour
Lane 5 : Hep3B lysate (control)
Lane 6 : Hep3B lysate, after stimulation with 2 ng/ml of TGF-ß for one hour
Lane 7 : HEK 293 lysate (control)
Predicted band size: 48 kDa
Image courtesy of Ying Zhang, NIH, CCR, Bethesda, MD.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (54)
ab51451 は 54 報の論文で使用されています。
- Baik JE et al. TGF-β1 mediates pathologic changes of secondary lymphedema by promoting fibrosis and inflammation. Clin Transl Med 12:e758 (2022). PubMed: 35652284
- Emmert ME et al. Sex-specific role of myostatin signaling in neonatal muscle growth, denervation atrophy, and neuromuscular contractures. Elife 11:N/A (2022). PubMed: 36314781
- Silvestro M et al. The Nonproteolytic Intracellular Domain of Membrane-Type 1 Matrix Metalloproteinase Coordinately Modulates Abdominal Aortic Aneurysm and Atherosclerosis in Mice-Brief Report. Arterioscler Thromb Vasc Biol 42:1244-1253 (2022). PubMed: 36073351
- Walewska E et al. The Interaction Between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum. Front Endocrinol (Lausanne) 10:667 (2019). PubMed: 31632347
- Ursoli Ferreira F et al. Endothelial Cells Tissue-Specific Origins Affects Their Responsiveness to TGF-ß2 during Endothelial-to-Mesenchymal Transition. Int J Mol Sci 20:N/A (2019). PubMed: 30678183