Anti-Smad1 抗体 (ab63356)
Key features and details
- Rabbit polyclonal to Smad1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Smad1 antibody
Smad1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Smad1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic non-phosphopeptide derived from human Smad1 around the phosphorylation site of serine 187.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63356の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
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| ICC/IF |
1/500 - 1/1000.
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| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa). |
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ICC/IF
1/500 - 1/1000. |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. -
組織特異性
Ubiquitous. Highest expression seen in the heart and skeletal muscle. -
配列類似性
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
翻訳後修飾
Phosphorylated on serine by BMP type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4086 Human
- Omim: 601595 Human
- SwissProt: Q15797 Human
- Unigene: 604588 Human
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別名
- BSP-1 antibody
- BSP1 antibody
- HsMAD1 antibody
see all
画像
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Western blot - Anti-Smad1 antibody (ab63356)All lanes : Anti-Smad1 antibody (ab63356) at 1/500 dilution
Lane 1 : extracts from 293 cell line
Lane 2 : extracts from 293 cell line, with immunizing peptide at 5 µg
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug. -
Immunocytochemistry/ Immunofluorescence - Anti-Smad1 antibody (ab63356)Immunofluorescence analysis of Smad1 expression in HeLa cells, using 1/500 ab63356 -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad1 antibody (ab63356)ab63356 (1µg/ml) staining SMAD1 in human stomach using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab63356 は 9 報の論文で使用されています。
- Huang F et al. BMP2 signalling activation enhances bone metastases of non-small cell lung cancer. J Cell Mol Med 24:10768-10784 (2020). PubMed: 32750747
- Kim MS & Jin W TrkB Inhibits the BMP Signaling-Mediated Growth Inhibition of Cancer Cells. Cancers (Basel) 12:N/A (2020). PubMed: 32731498
- Ahmad N et al. MicroRNA-672-5p Identified during Weaning Reverses Osteopenia and Sarcopenia in Ovariectomized Mice. Mol Ther Nucleic Acids 14:536-549 (2019). PubMed: 30769134
- Zhang J & Liu F Expression of BMP-4 and Smad1 in patients with Hirschsprung disease and its clinical significance. Exp Ther Med 18:225-229 (2019). PubMed: 31258657
- Ma YS et al. miR-30 Family Reduction Maintains Self-Renewal and Promotes Tumorigenesis in NSCLC-Initiating Cells by Targeting Oncogene TM4SF1. Mol Ther 26:2751-2765 (2018). PubMed: 30301667
- Wang L et al. Green Tea Catechins Effectively Altered Hepatic Fibrogenesis in Rats by Inhibiting ERK and Smad1/2 Phosphorylation. J Agric Food Chem N/A:N/A (2018). PubMed: 30424599
- Dudakovic A et al. Enhancer of Zeste Homolog 2 Inhibition Stimulates Bone Formation and Mitigates Bone Loss Caused by Ovariectomy in Skeletally Mature Mice. J Biol Chem 291:24594-24606 (2016). PubMed: 27758858
- Dharmarajan S et al. Bone morphogenetic protein 7 regulates reactive gliosis in retinal astrocytes and Müller glia. Mol Vis 20:1085-108 (2014). Mouse . PubMed: 25253985
- Maioli M et al. Hyaluronan esters drive Smad gene expression and signaling enhancing cardiogenesis in mouse embryonic and human mesenchymal stem cells. PLoS One 5:e15151 (2010). WB, ICC/IF ; Human, Mouse . PubMed: 21152044