Anti-SMAD 1/5/8/9 抗体 (ab13723)
Key features and details
- Rabbit polyclonal to SMAD 1/5/8/9
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SMAD 1/5/8/9 antibody -
製品の詳細
Rabbit polyclonal to SMAD 1/5/8/9 -
由来種
Rabbit -
特異性
ab13723 may cross-react with SMAD2 or SMAD3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to amino acids 18-32 and amino acids 315-329 of Human SMAD1. These sequences are identical in human SMAD5 and SMAD8/9.
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ポジティブ･コントロール
- A549 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13723の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 51 kDa.
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| 特記事項 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 51 kDa. |
ターゲット情報
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関連性
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs, common-mediator SMAD and inhibitory SMADs. SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology. SMAD8 and SMAD9 are used as alternate names for the same protein. Human SMAD1 is a 465 amino acid protein, human SMAD5 is a 465 amino acid and human SMAD9 is a 430 amino acid protein. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 4086 Human
- Entrez Gene: 4090 Human
- Entrez Gene: 4093 Human
- Omim: 601595 Human
- Omim: 603110 Human
- Omim: 603295 Human
- SwissProt: O15198 Human
- SwissProt: Q15797 Human
see all -
別名
- DPC4 antibody
- Madh6 antibody
- MADH9 antibody
see all
画像
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Western blot - Anti-SMAD 1/5/8/9 antibody (ab13723)
Western blot analysis of SMAD1/5/8/9 in 15 ugs of A549 cell lysate using ab13723 at 0.5 ug/ml.
Western blot analysis of SMAD1/5/8/9 in 15 ugs of A549 cell lysate using ab13723 at 0.5 ug/ml.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab13723 は 6 報の論文で使用されています。
- Rosato BE et al. SEC23B Loss-of-Function Suppresses Hepcidin Expression by Impairing Glycosylation Pathway in Human Hepatic Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35163229
- Cai C et al. Msx2 plays an important role in BMP6-induced osteogenic differentiation of two mesenchymal cell lines: C3H10T1/2 and C2C12. Regen Ther 14:245-251 (2020). PubMed: 32455154
- Andolfo I et al. The BMP-SMAD pathway mediates the impaired hepatic iron metabolism associated with the ERFE-A260S variant. Am J Hematol 94:1227-1235 (2019). PubMed: 31400017
- Gao Y et al. Propofol inhibits pancreatic cancer progress under hypoxia via ADAM8. J Hepatobiliary Pancreat Sci 26:219-226 (2019). PubMed: 30945470
- Zhu Z et al. Aucubin suppresses Titanium particles-mediated apoptosis of MC3T3-E1 cells and facilitates osteogenesis by affecting the BMP2/Smads/RunX2 signaling pathway. Mol Med Rep 18:2561-2570 (2018). PubMed: 30015916
- Scimeca M et al. Bone Morphogenetic Proteins and myostatin pathways: key mediator of human sarcopenia. J Transl Med 15:34 (2017). PubMed: 28202082