AB13723

Anti-SMAD 1/5/8/9 抗体

Name: Anti-SMAD 1/5/8/9 抗体 (ab13723) | Abcam
Brand: Abcam Limited
SKU: AB13723
Price: 67000 JPY
Availability: InStock

Anti-SMAD 1/5/8/9 antibody

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(11 Publications)

Rabbit Polyclonal SMAD1 antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD1 aa 1-50.

別名を表示する

BSP1, MADH1, MADR1, SMAD1, SMAD family member 1, SMAD 1, hSMAD1, JV4-1, Mad-related protein 1, Mothers against decapentaplegic homolog 1, Transforming growth factor-beta-signaling protein 1, MAD homolog 1, Mothers against DPP homolog 1, BSP-1

1 Images

Unknown

Western blot - Anti-SMAD 1/5/8/9 antibody (AB13723)

Western blot analysis of SMAD1/5/8/9 in 15 ugs of A549 cell lysate using ab13723 at 0.5 ug/ml. Western blot analysis of SMAD1/5/8/9 in 15 ugs of A549 cell lysate using ab13723 at 0.5 ug/ml.

All lanes:

Western blot - Anti-SMAD 1/5/8/9 antibody (ab13723)

Predicted band size: 52 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

Synthetic Peptide within Human SMAD1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q15797

特異性

ab13723 may cross-react with SMAD2 or SMAD3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

バッファー組成

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SMAD 1/5/8/9 also known by the genes SMAD1 SMAD5 SMAD8 and SMAD9 are integral proteins in the transforming growth factor-beta (TGF-β) signaling pathway. These proteins function as transcriptional modulators once activated. The molecular weight of SMAD1 SMAD5 and SMAD8 ranges from approximately 60 to 65 kDa. These proteins are expressed in various tissues and are especially active in bone cartilage and the vascular system. They act by transmitting extracellular signals from TGF-β superfamily ligands to the nucleus leading to gene expression changes.

Biological function summary

SMAD1/5/8/9 proteins play essential roles in processes like cellular proliferation differentiation and apoptosis. These proteins typically form complexes with other SMAD proteins such as SMAD4 upon activation by type I receptor kinases. The formation of these complexes is important for their translocation to the nucleus where they regulate target gene expression. This regulation is key in controlling pivotal developmental and homeostatic processes across different cell types.

Pathways

SMAD1/5/8/9 proteins are central components of the bone morphogenetic protein (BMP) signaling pathway. They mediate the signal transduction from BMPs which are important for bone development and embryogenesis. These proteins interact with other signaling molecules such as BMP receptors and various co-SMADs like SMAD4 to relay and modulate signals effectively. Through these interactions SMAD1/5/8/9 function within a well-defined network ensuring precise cellular responses to BMP stimuli.

SMAD1/5/8/9 proteins show significant involvement in conditions like cancer and pulmonary arterial hypertension (PAH). Aberrant BMP signaling through dysfunctions in these SMAD proteins has been linked to tumorigenesis where abnormal cell proliferation is a hallmark. Additionally alterations in SMAD1/5/8/9 activity are associated with PAH a condition characterized by high blood pressure in the pulmonary arteries. In these diseases SMAD4 often acts as a partner in complex formation with SMAD1/5/8/9 underlining its importance in maintaining normal signaling processes.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transcriptional modulator that plays a role in various cellular processes, including embryonic development, cell differentiation, and tissue homeostasis (PubMed : 9335504). Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form a heteromeric complex which translocates into the nucleus acting as transcription factor (PubMed : 33667543). In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network (PubMed : 33667543). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Positively regulates BMP4-induced expression of odontogenic development regulator MSX1 following IPO7-mediated nuclear import (By similarity).

See full target information SMAD1

追加のターゲット

SMAD9,SMAD5

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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2036 PubMed40021625

2025

Bone morphogenetic protein (BMP) signaling determines neuroblastoma cell fate and sensitivity to retinoic acid.

Applications

Unspecified application

Species

Unspecified reactive species

Min Pan,Yinwen Zhang,William C Wright,Xueying Liu,Barbara Passaia,Duane Currier,Jonathan Low,Richard H Chapple,Jacob A Steele,Jon P Connelly,Bensheng Ju,Emily Plyler,Meifen Lu,Allister J Loughran,Lei Yang,Brian J Abraham,Shondra M Pruett-Miller,Burgess Freeman,George E Campbell,Michael A Dyer,Taosheng Chen,Elizabeth Stewart,Selene Koo,Heather Sheppard,John Easton,Paul Geeleher

Journal of orthopaedic surgery and research 20:70 PubMed39833827

2025

Deer antler velvet (Cervus elaphus sibiricus) promotes fracture healing via partial BMP2-Smad mediated osteoblast differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Hyang Gu,Min-Young Chae,Jeong June Choi,Thanh Chu Duc,Chang Gue Son,Eun-Jung Lee

American journal of hematology 100:52-65 PubMed39558179

2024

RAS signaling pathway is essential in regulating PIEZO1-mediated hepatic iron overload in dehydrated hereditary stomatocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Eleni Rosato,Vanessa D'Onofrio,Roberta Marra,Antonella Nostroso,Federica Maria Esposito,Anthony Iscaro,Vito Alessandro Lasorsa,Mario Capasso,Achille Iolascon,Roberta Russo,Immacolata Andolfo

Respiratory research 25:316 PubMed39160536

2024

Levosimendan mediates the BMP/Smad axis through upregulation of circUSP34-targeted miR-1298 to alleviate pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Meng,Linhong Song,Hui Wang,Gang Wang,Gengxu Zhou

Cellular and molecular biology (Noisy-le-Grand, France) 70:85-91 PubMed38836676

2024

The combination of tetramethylpyrazine and vitamin A acid in the treatment of skin photoaging by activating the HIF-1α pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weijun Chen,Jianyong Fan,Xuelian Zou,Yuansheng Wu

International journal of molecular sciences 23: PubMed35163229

2022

SEC23B Loss-of-Function Suppresses Hepcidin Expression by Impairing Glycosylation Pathway in Human Hepatic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Eleni Rosato,Roberta Marra,Vanessa D'Onofrio,Federica Del Giudice,Simone Della Monica,Achille Iolascon,Immacolata Andolfo,Roberta Russo

Regenerative therapy 14:245-251 PubMed32455154

2020

Msx2 plays an important role in BMP6-induced osteogenic differentiation of two mesenchymal cell lines: C3H10T1/2 and C2C12.

Applications

Unspecified application

Species

Unspecified reactive species

Chuan Cai,Jing Wang,Na Huo,Li Wen,Peng Xue,Ye Huang

American journal of hematology 94:1227-1235 PubMed31400017

2019

The BMP-SMAD pathway mediates the impaired hepatic iron metabolism associated with the ERFE-A260S variant.

Applications

Unspecified application

Species

Unspecified reactive species

Immacolata Andolfo,Barbara Eleni Rosato,Roberta Marra,Gianluca De Rosa,Francesco Manna,Antonella Gambale,Achille Iolascon,Roberta Russo

Journal of hepato-biliary-pancreatic sciences 26:219-226 PubMed30945470

2019

Propofol inhibits pancreatic cancer progress under hypoxia via ADAM8.

Applications

Unspecified application

Species

Unspecified reactive species

Yutong Gao,Xiangdi Yu,Fangxiang Zhang,Jing Dai

Molecular medicine reports 18:2561-2570 PubMed30015916

2018

Aucubin suppresses Titanium particles‑mediated apoptosis of MC3T3‑E1 cells and facilitates osteogenesis by affecting the BMP2/Smads/RunX2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ziguan Zhu,Qingping Xie,Yazeng Huang,Shuijun Zhang,Yu Chen

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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For licensing inquiries, please contact partnerships@abcam.com

Anti-SMAD 1/5/8/9 抗体

Anti-SMAD 1/5/8/9 antibody

Western blot - Anti-SMAD 1/5/8/9 antibody (AB13723)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (11)

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