Anti-Smac/Diablo 抗体 [EPR10934] (ab174284)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac/Diablo antibody [EPR10934] (ab174284)](https://www.abcam.co.jp/ps/products/174/ab174284/Images/ab174284-199512-ab174284IHC2.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac/Diablo antibody [EPR10934] (ab174284)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10934] to Smac/Diablo
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Smac/Diablo antibody [EPR10934]
Smac/Diablo 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10934] to Smac/Diablo -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
-
免疫原
Recombinant fragment within Human Smac/ Diablo. The exact sequence is proprietary.
Database link: Q9NR28 -
ポジティブ･コントロール
- HeLa, MCF7, Jurkat, 293T and Human testis lysates; Human heart and testis tissue.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR10934 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab174284の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 27 kDa.
|
| 特記事項 |
|---|
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 27 kDa. |
ターゲット情報
-
機能
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). -
組織特異性
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. -
ドメイン
The mature N-terminus mediates interaction with XIAP. -
細胞内局在
Mitochondrion. Released into the cytosol when cells undergo apoptosis. - Information by UniProt
-
参照データベース
- Entrez Gene: 56616 Human
- Entrez Gene: 66593 Mouse
- Entrez Gene: 288753 Rat
- Omim: 605219 Human
- SwissProt: Q9NR28 Human
- SwissProt: Q9JIQ3 Mouse
- Unigene: 169611 Human
- Unigene: 46716 Mouse
-
別名
- 0610041G12Rik antibody
- DBLOH_HUMAN antibody
- DBOH antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac/Diablo antibody [EPR10934] (ab174284)
Immunohistochemical analysis of paraffinn embedded Human testis tissue labeling Smac / Diablo with ab174284 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac/Diablo antibody [EPR10934] (ab174284)](https://www.abcam.co.jp/ps/products/174/ab174284/Images/ab174284-199510-ab174284IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac/Diablo antibody [EPR10934] (ab174284)Immunohistochemical analysis of paraffinn embedded Human heart tissue labeling Smac / Diablo with ab174284 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Western blot - Anti-Smac/Diablo antibody [EPR10934] (ab174284)](https://www.abcam.co.jp/ps/products/174/ab174284/Images/ab174284-199508-ab174284WB.jpg)
Western blot - Anti-Smac/Diablo antibody [EPR10934] (ab174284)All lanes : Anti-Smac/Diablo antibody [EPR10934] (ab174284) at 1/1000 dilution
Lane 1 : HeLa cell line lysate
Lane 2 : MCF7 cell line lysate
Lane 3 : Jurkat cell line lysate
Lane 4 : 293T cell line lysate
Lane 5 : Human testis tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa -
![Anti-Smac/Diablo antibody [EPR10934] (ab174284)](https://www.abcam.co.jp/ps/products/174/ab174284/Images/ab174284-4-benefits-of-recombinant-antibodies.png)
Anti-Smac/Diablo antibody [EPR10934] (ab174284)
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab174284 は 1 報の論文で使用されています。
- Li Y et al. AREL1 resists the apoptosis induced by TGF-β by inhibiting SMAC in vascular endothelial cells. J Biochem Mol Toxicol 37:e23439 (2023). PubMed: 37522329