Anti-Smac/Diablo 抗体 (ab8115)
Key features and details
- Rabbit polyclonal to Smac/Diablo
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Smac/Diablo antibody
Smac/Diablo 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Smac/Diablo -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Mouse Smac/Diablo aa 200-300.
Database link: Q9JIQ3 -
ポジティブ･コントロール
- WB: Mouse and rat heart tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
DEAE-Chromatography -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8115の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml.
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| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. |
ターゲット情報
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機能
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). -
組織特異性
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. -
ドメイン
The mature N-terminus mediates interaction with XIAP. -
細胞内局在
Mitochondrion. Released into the cytosol when cells undergo apoptosis. - Information by UniProt
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参照データベース
- Entrez Gene: 66593 Mouse
- Entrez Gene: 288753 Rat
- SwissProt: Q9JIQ3 Mouse
- Unigene: 46716 Mouse
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別名
- 0610041G12Rik antibody
- DBLOH_HUMAN antibody
- DBOH antibody
see all
画像
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Western blot - Anti-Smac/Diablo antibody (ab8115)All lanes : Anti-Smac/Diablo antibody (ab8115) at 1 µg/ml
Lane 1 : Mouse heart tissue lysate with absence of blocking peptide
Lane 2 : Mouse heart tissue lysate with presence of blocking peptide
Lane 3 : Rat heart tissue lysate with absence of blocking peptide
Observed band size: 25 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (8)
ab8115 は 8 報の論文で使用されています。
- Udoh US et al. Tumor-Suppressor Role of the α1-Na/K-ATPase Signalosome in NASH Related Hepatocellular Carcinoma. Int J Mol Sci 23:N/A (2022). PubMed: 35806364
- Takahashi J et al. Interleukin-22 Directly Activates Myocardial STAT3 (Signal Transducer and Activator of Transcription-3) Signaling Pathway and Prevents Myocardial Ischemia Reperfusion Injury. J Am Heart Assoc 9:e014814 (2020). PubMed: 32301368
- Wischhof L et al. A disease-associated Aifm1 variant induces severe myopathy in knockin mice. Mol Metab N/A:N/A (2018). PubMed: 29780003
- Liu S et al. Silencing Livin improved the sensitivity of colon cancer cells to 5-fluorouracil by regulating crosstalk between apoptosis and autophagy. Oncol Lett 15:7707-7715 (2018). PubMed: 29740490
- Shephard F et al. Analysis of Mitochondrial haemoglobin in Parkinson's disease brain. Mitochondrion 29:45-52 (2016). WB . PubMed: 27181046
- Admoni-Elisha L et al. Novel Biomarker Proteins in Chronic Lymphocytic Leukemia: Impact on Diagnosis, Prognosis and Treatment. PLoS One 11:e0148500 (2016). Human . PubMed: 27078856
- Shephard F et al. A mitochondrial location for haemoglobins--dynamic distribution in ageing and Parkinson's disease. Mitochondrion 14:64-72 (2014). WB ; Mouse . PubMed: 24333691
- Heazell AE et al. Altered expression of regulators of caspase activity within trophoblast of normal pregnancies and pregnancies complicated by preeclampsia. Reprod Sci 15:1034-43 (2008). WB ; Human . PubMed: 19088373
